Improving the Acid Resistance of Tannase TanBLp (AB379685) from Lactobacillus plantarum ATCC14917(T) by Site-Specific Mutagenesis
文献类型： 外文期刊
作者： Pan, Hu 1 ; Zhan, Jingjing 1 ; Yang, Hui 1 ; Wang, Chong 1 ; Liu, Huhu 1 ; Zhou, Hui 3 ; Zhou, Haiyan 1 ; Lu, Xiangyang 1 ;
作者机构： 1.Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China
2.Inst Agr Product Qual Standard & Testing Res, Tibet Acad Agr & Anim Husb Sci, Lhasa, Peoples R China
3.Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China
关键词： Tannase; TanBLp; Acid resistance; Catalytic activity; Site-specific mutagenesis
期刊名称：INDIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.461； 五年影响因子：2.259 ）
ISSN： 0046-8991
年卷期：
页码：
收录情况： SCI
摘要： Tannin acyl hydrolase referred commonly as tannase catalyzes the hydrolysis of the galloyl ester bond of tannin to release gallic acid. The tannase TanBLp which cloned from Lactobacillus plantarum ATCC14917(T) has high activity in the pH range (7.0-9.0) at 40 degrees C, it would be detrimental to the utilization at acidic environment. The catalytic sites and stability of TanBLp were analyzed using bioinformatics and site-specific mutagenesis. The results reiterated that the amino acid residues Ala164, Lys343, Glu357, Asp421 and His451 had played an important role in maintaining the activity. The optimum pH of mutants V75A, G77A, N94A, A164S and F243A were shifted from 8.0 to 6.0, and mutant V75A has the highest pH stability and activity at acidic conditions than other mutants, which was more suitable for industrial application to manufacture gallic acid. This study was of great significance to promote the industrialization and efficient utilization of tannase TanBLp.
- 相关文献
作者其他论文 更多>>
-
Antibacterial mechanism of vitamin C against Staphylococcus aureus, reduction of toxicity of metabolites and its application in pasteurized milk
作者：Tian, Lei;Huang, Chunyang;Liu, Xing;Zhang, Yuxin;Zhang, Chunling;Wang, Xin;Zhou, Ting;Yu, Ying;Pan, Hu;Dai, Yanna;Pu, Jifeng;Fu, Chengyu;Zhang, Pengfei
关键词：Vitamin C; Staphylococcus aureus; Antibacterial mechanism; Pasteurized milk
-
Methylotrophic yeasts for chemicals production using methanol as substrate: Current status, challenges, and strategies
作者：Duan, Xiyu;Wang, Zhi;Wang, Chong;Yang, Xiaona;Liu, Huhu;Lu, Xiangyang;Tian, Yun;Li, Changzhu;Li, Peiwang;Tian, Yun;Wang, Chong;Yang, Xiaona;Liu, Huhu;Lu, Xiangyang;Li, Changzhu;Li, Peiwang;Tian, Yun;Tian, Yun
关键词：Methylotrophic yeasts; Methanol; C1 substrate; Metabolic engineering; Green biomanufacturing
-
Targeted subcellular localization of a novel fungicidal compound N-(naphthalen-1-yl) phenazine-1-carboxamide inhibiting Rhizoctonia solani
作者：Liu, Shuangqing;Wen, Ding;Jia, Wenhui;Liao, Xiaolan;Zhang, Ya;Wang, Chong;Pan, Hu
关键词：Rhizoctonia solani; N-(naphthalen-1-yl)phenazine-1-carboxamide; Polyclonal antibody; Subcellular localization
-
Enhancing Cordycepin Biosynthesis in Yarrowia lipolytica via Lipid Droplets Compartmentalization Engineering and Optimized Fermentation Strategies
作者：Duan, Xi-Yu;Wang, Zhi;Song, Liping;Jin, Qing;Yang, Xiao-Na;Liu, Hu-Hu;Wang, Chong;Lu, Xiangyang;Tian, Yun;Ji, Xiao-Jun;Tian, Yun
关键词：cordycepin;
Yarrowia lipolytica ; lipid droplets; compartmentalization; fermentationengineering -
Bifunctional nanobody facilitates a colorimetric and fluorescent dual-mode immunoassay of Staphylococcal enterotoxin A
作者：Zhang, Yao;Liu, Di;Tian, Yudong;Li, Min;Li, Yuhuan;Zhang, Min;Wang, Xin;Zhou, Ting;Zhao, Qin;Yu, Ying;Pan, Hu;Dai, Yanna;Dawa, Zhuoma;Zheng, Wanxiang
关键词：Nanobody; Bifunctional protein; Dual-mode immunoassay; Natural fluorescent product
-
Antibacterial mechanism of lysine against Escherichia coli O157:H7, its action on lettuce and in reducing the severity of murine colitis
作者：Tian, Lei;Ma, Luyao;Tian, Yudong;Zhang, Chunling;Wang, Xin;Zhou, Ting;Pan, Hu;Dai, Yanna;Pu, Jifeng;Fu, Chengyu;Zhang, Pengfei
关键词：Lysine; Escherichia coli O157:H7; Antibacterial mechanism; Lettuce; Colitis
-
Pararhizobium qamdonense sp. nov., Isolated from an Alpine Soil in Tibet and the Reclassification of Rhizobium gei Shi et al. 2016 as Pararhizobium gei comb. nov.
作者：Zhang, Yi-fan;Dai, Yan-na;Pu, Ji-feng;Pan, Hu;Liu, Hu-hu;Pan, Hu;Tian, Yun;Zhang, Yi-fan;Dai, Yan-na;Pu, Ji-feng;Pan, Hu;Yang, Xiao-feng
关键词：
