Antibacterial mechanism of lysine against Escherichia coli O157:H7, its action on lettuce and in reducing the severity of murine colitis
文献类型： 外文期刊
作者： Tian, Lei 1 ; Ma, Luyao 1 ; Tian, Yudong 1 ; Zhou, Ting 2 ; Zhang, Chunling 1 ; Pan, Hu 3 ; Dai, Yanna 3 ; Pu, Jifeng 3 ; Fu, Chengyu 4 ; Zhang, Pengfei 4 ; Wang, Xin 1 ;
作者机构： 1.Northwest A&F Univ, Coll Food Sci & Engn, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China
2.Northwest A&F Univ, Coll Vet Med, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China
3.Tibet Acad Agr & Anim Husb Sci, Inst Agr Qual Stand & Testing, 130 Jinzhu West Rd, Lhasa 850032, Tibet, Peoples R China
4.Xian Polytech Univ, Sch Text Sci & Engn, 19 Jinhua Rd, Xian 710048, Shaanxi, Peoples R China
关键词： Lysine; Escherichia coli O157:H7; Antibacterial mechanism; Lettuce; Colitis
期刊名称：FOOD MICROBIOLOGY （ 影响因子：4.6； 五年影响因子：5.1 ）
ISSN： 0740-0020
年卷期： 2025 年 129 卷
页码：
收录情况： SCI
摘要： The aim of the present study was to evaluate the mechanism of antimicrobial action of Lysine (Lys) against Escherichia coli (E. coli) O157:H7. Lys alters the permeability and morphology of bacterial cell membranes, leading to leakage of intracellular material. Using agarose gel electrophoresis and sodium dodecyl sulfatepolyacrylamide gel electrophoresis(SDS-PAGE), it was found that high concentrations of Lys disrupted the DNA and protein primary structure of E. coli O157:H7. Lys also inhibited the ability of E. coli biofilm formation in a concentration-dependent manner. Subinhibitory concentration of Lys reduces the swimming and swarming ability of E. coli O157:H7 and its ability to adhere and to invade Caco-2 cells. The use of Lys on lettuce inoculated with E. coli O157:H7 showed good disinfection efficiency. Further studies revealed that Lys had both preventive and therapeutic effects on the severity of colitis induced by E. coli O157:H7 infected mice, and that the preventive effect was greater than the therapeutic effect.
- 相关文献
作者其他论文 更多>>
-
Antibacterial mechanism of vitamin C against Staphylococcus aureus, reduction of toxicity of metabolites and its application in pasteurized milk
作者：Tian, Lei;Huang, Chunyang;Liu, Xing;Zhang, Yuxin;Zhang, Chunling;Wang, Xin;Zhou, Ting;Yu, Ying;Pan, Hu;Dai, Yanna;Pu, Jifeng;Fu, Chengyu;Zhang, Pengfei
关键词：Vitamin C; Staphylococcus aureus; Antibacterial mechanism; Pasteurized milk
-
Targeted subcellular localization of a novel fungicidal compound N-(naphthalen-1-yl) phenazine-1-carboxamide inhibiting Rhizoctonia solani
作者：Liu, Shuangqing;Wen, Ding;Jia, Wenhui;Liao, Xiaolan;Zhang, Ya;Wang, Chong;Pan, Hu
关键词：Rhizoctonia solani; N-(naphthalen-1-yl)phenazine-1-carboxamide; Polyclonal antibody; Subcellular localization
-
Mechanisms of altitude-driven mycotoxin variation in stored wheat: a metabolomic and transcriptomic approach
作者：Wei, Na;Pu, Jifeng
关键词：Alternaria; TeA; Xizang; Metabolome; Transcriptome; Amino acid; Toxin biosynthesis pathway; PacC
-
Bifunctional nanobody facilitates a colorimetric and fluorescent dual-mode immunoassay of Staphylococcal enterotoxin A
作者：Zhang, Yao;Liu, Di;Tian, Yudong;Li, Min;Li, Yuhuan;Zhang, Min;Wang, Xin;Zhou, Ting;Zhao, Qin;Yu, Ying;Pan, Hu;Dai, Yanna;Dawa, Zhuoma;Zheng, Wanxiang
关键词：Nanobody; Bifunctional protein; Dual-mode immunoassay; Natural fluorescent product
-
Pararhizobium qamdonense sp. nov., Isolated from an Alpine Soil in Tibet and the Reclassification of Rhizobium gei Shi et al. 2016 as Pararhizobium gei comb. nov.
作者：Zhang, Yi-fan;Dai, Yan-na;Pu, Ji-feng;Pan, Hu;Liu, Hu-hu;Pan, Hu;Tian, Yun;Zhang, Yi-fan;Dai, Yan-na;Pu, Ji-feng;Pan, Hu;Yang, Xiao-feng
关键词：
-
Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet
作者：Pan, Hu;Dai, Yanna;Pan, Hu;Zhu, Beike;Bu, Wenbin;Lu, Xiangyang;Liu, Huhu;Tian, Yun;Li, Jin;Zhou, Ziqiong
关键词：Azospirillum; novel taxa; iprodione; degradation pathway; bioremediation
-
Acinetobacter tibetensis sp. nov., Isolated from a Soil Under a Greenhouse in Tibet
作者：Pan, Hu;Li, Jin;Liu, Hu-Hu;Lu, Xiang-Yang;Tian, Yun;Pan, Hu;Zhang, Yi-Fan;Pan, Hu;Zhang, Yi-Fan;Li, Jin
关键词：
