Bifunctional nanobody facilitates a colorimetric and fluorescent dual-mode immunoassay of Staphylococcal enterotoxin A
文献类型： 外文期刊
作者： Zhang, Yao 1 ; Liu, Di 1 ; Tian, Yudong 1 ; Li, Min 1 ; Li, Yuhuan 1 ; Zhou, Ting 2 ; Zhao, Qin 2 ; Zhang, Min 1 ; Yu, Ying 3 ; Pan, Hu 4 ; Dai, Yanna 4 ; Dawa, Zhuoma 4 ; Zheng, Wanxiang 5 ; Wang, Xin 1 ;
作者机构： 1.Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China
2.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
3.China Agr Univ, Coll Anim Sci & Technol, Key Lab Agr Anim Genet & Breeding, Minist Agr,Natl Engn Lab Anim Breeding, Beijing 100193, Peoples R China
4.Tibet Acad Agr & Anim Husb Sci, Inst Agr Qual Stand & Testing, Lhasa 850032, Xizang, Peoples R China
5.Fourth Mil Med Univ, Xijing Hosp, Dept Urol, 127 Changle West Rd, Xian 710065, Shaanxi, Peoples R China
关键词： Nanobody; Bifunctional protein; Dual-mode immunoassay; Natural fluorescent product
期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）
ISSN： 0308-8146
年卷期： 2025 年 467 卷
页码：
收录情况： SCI
摘要： Immunoassay is a diagnostic tool based on the specific binding of antibodies and antigens with widespread applications. Nonetheless, several research obstacles, like poor specific antibodies, the poisonous reagents and unstable results, still remain challenges. Herein, we innovatively reported a colorimetric and fluorescent dualmode immunoassay based on the bifunctional nanobody for SEA detection. Benefiting from the advantages of nanobodies, the bifunctional protein with both recognition and catalysis was built to identify and catalyze with efficiency to generate the first colorimetric signal. Meanwhile, the introduction of quinine as the natural source of the second fluorescent signal greatly improved the stability and safety of detection. In addition, the proposed method was successfully applied to detecting SEA in food samples with high accuracy and stability. This study integrated the bifunctional nanobody with eco-friendly fluorescent product to provide a specific and green platform for the detection of foodborne toxins.
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