您好，欢迎访问西藏自治区农牧科学院 机构知识库！

Mechanisms of altitude-driven mycotoxin variation in stored wheat: a metabolomic and transcriptomic approach

文献类型： 外文期刊

作者： Wei, Na 1 ; Pu, Jifeng 1 ;

作者机构： 1.Xizang Acad Agr & Anim Husb Sci, Inst Agr Prod Qual Stand & Testing Res, Lhasa 850032, Peoples R China

关键词： Alternaria; TeA; Xizang; Metabolome; Transcriptome; Amino acid; Toxin biosynthesis pathway; PacC

期刊名称：BMC MICROBIOLOGY （ 影响因子：4.2； 五年影响因子：4.8 ）

ISSN： 1471-2180

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCI

摘要： The Alternaria fungus represents a significant threat to grain storage safety due to its production of mycotoxins, which pose serious risks to human health. This study explores the toxin profile of 153 stored wheat samples collected from five distinct regions in Xizang , analyzed by ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Our findings reveal that tenuazonic acid (TeA) is the predominant toxin detected in wheat (Triticum aestivum L.), with toxin levels exhibiting a significant negative correlation to altitude. To elucidate the molecular mechanisms underlying the differential toxigenic profiles of Alternaria alternata strains from various altitudes, integration of metabolomic and transcriptomic analyses was performed. Alternaria strains were categorized based on their toxin-producing capacity as high toxin-producing (HT), medium toxin-producing (MT), and low toxin-producing (LT) strains. The data indicate that altitude influences the activity of amino acid and carbohydrate metabolism, both of which are critical to the biosynthesis of Alternaria toxins. Co-expression analysis identified nine candidate structural genes and four key transcription factors that regulate the toxin biosynthesis pathway. Notably, the PacC transcription factor emerged as a central regulator of toxin production in response to altitude. Functional validation using a PacC knockout mutant confirmed a reduction in toxin production, along with downregulation of the associated biosynthetic genes, particularly those involved in TeA synthesis. This integrated approach provides new insights into the molecular and metabolic mechanisms driving the variation in toxin production, offering potential targets for mitigating mycotoxin contamination in stored grain.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：西藏自治区农牧科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台