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Cloning and analysis of the Oswc-1 gene encoding a putative blue light photoreceptor from Ophiocordyceps sinensis

文献类型: 外文期刊

作者: Yang, Tao 1 ; Xiong, Weiping 3 ; Dong, Caihong 1 ;

作者机构: 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Inst Vegetable Sci, Lhasa 850002, Tibet, Peoples R China

关键词: Chinese caterpillar fungus;Light-inducible;Quantitative real-time PCR;White collar-1

期刊名称:MYCOSCIENCE ( 影响因子:1.223; 五年影响因子:1.266 )

ISSN: 1340-3540

年卷期: 2014 年 55 卷 4 期

页码:

收录情况: SCI

摘要: Light plays an important role during both sexual and asexual growth of Ophiocordyceps sinensis, one of the best-known traditional Chinese medicines. In the present study, we cloned Oswc-1, the homologue of the blue light photoreceptor Ncwc-1 of Neurospora crassa, from O. sinensis by Hi-tail polymerase chain reaction. The deduced amino acid sequence of Oswc-1 contains the similar function domains as NcWC-1 including transcriptional activation, LOV (Light, Oxygen, or Voltage), PAS (Per-Arnt-Sim) and Zinc Finger domains. Phylogenetic analysis based on fungal WC-1 supported OsWC-1 was a blue light receptor. The expression of Oswc-1 messenger RNA was up-regulated upon irradiation significantly. (C) 2013 The Mycological Society of Japan. Published by Elsevier B. V. All rights reserved.

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