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The expression spectrum of yak epididymal epithelial cells reveals the functional diversity of caput, corpus and cauda regions

文献类型： 外文期刊

作者： Pan, Meilan 1 ; Luo, Xiaofeng 1 ; Zhang, Zhenzhen 1 ; Li, Jingjing 1 ; Shahzad, Khuram 2 ; Danba, Zhaxi 3 ; Caiwang, Gongbu 4 ; Chilie, Wangmu 3 ; Chen, Xiaoying 5 ; Zhao, Wangsheng 1 ;

作者机构： 1.Southwest Univ Sci & Technol, Coll Life Sci & Engn, Mianyang 621000, Sichuan, Peoples R China

2.COMSATS Univ Islamabad, Dept Biosci, Pk Rd, Islamabad 45550, Pakistan

3.Agr & Anim Husb Grass Ind, Sci & Technol Res & Promot Ctr, Naqu 852200, Tibet, Peoples R China

4.Tibet Naqu Municipal Agr & Rural Affairs Bur, Naqu 852000, Tibet, Peoples R China

5.Tibet Acad Agr & Anim Husb Sci, Inst Anim Sci, Lhasa 850009, Peoples R China

关键词： Epididymis epithelial cells; RNA-Seq; Differential gene expression

期刊名称：GENOMICS （ 影响因子：3.4； 五年影响因子：3.5 ）

ISSN： 0888-7543

年卷期： 2024 年 116 卷 5 期

页码：

收录情况： SCI

摘要： Sperm undergo a series of changes in the epididymis region before acquiring the ability to move and fertilize, and the identification of genes expressed in a region-specific manner in the epididymis provides a valuable insight into functional differences between regions. We collected epididymal tissue from three yaks and cultured epithelial cells from the caput, corpus and cauda regions of the yak epididymis using the tissue block method. RNA sequencing analysis (RNA-seq) technology was used to detect gene expression in yak epididymal caput, corpus and cauda epithelial cells. The results showed that the DEGs were highest in the caput vs. corpus comparison, and lowest in the corpus vs. cauda comparison. Six DEGs were verified by real-time fluorescence quantitative PCR (qRT-PCR), consistent with transcriptome sequencing results. The significantly enriched DNA replication pathway in the caput vs. corpus was coordinated with cell proliferation, while upregulated DEGs such as POLD1 and MCM4 were found in the DNA replication pathway. The AMPK signaling pathway was found significantly enriched in the caput vs cauda, suggesting its involvement in sperm maturation and capacitation. The TGF beta signaling pathway was screened in the corpus vs cauda and is crucial for mammalian reproductive regulation. Upregulated DEGs (TGFB3, INHBA, INHBB) are involved in the TGF beta signaling pathway. This study provides a reference for culturing yak epididymal epithelial cells in vitro, and elucidates the transcriptional profiles of epithelial cells in different segments of the epididymis, revealing the regulatory and functional differences between different segments, providing basic data for exploring the molecular mechanism of yak sperm maturation and improving the reproductive capacity of high-altitude mammals.

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