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Comparative transcriptome analysis in the caput segment of yak and cattleyak epididymis

文献类型： 外文期刊

作者： Adjei, Michael 1 ; Yan, Yan 2 ; Li, Chunhai 1 ; Pan, Cheng 1 ; Pan, Meilan 1 ; Wang, Peng 1 ; Li, Kerui 1 ; Shahzad, Khuram 4 ; Chen, Xiaoying 3 ; Zhao, Wangsheng 1 ;

作者机构： 1.Southwest Univ Sci & Technol, Sch Life Sci & Engn, Mianyang 621010, Sichuan, Peoples R China

2.Yanan Univ, Coll Life Sci, Yanan 716000, Shaanxi, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Inst Anim Sci, Lhasa 850009, Peoples R China

4.COMSATS Univ Islamabad, Dept Biosci, Pk Rd, Islamabad 45550, Pakistan

关键词： Cattleyak; Yak; Sperm maturation; RNA-Seq; DEGs

期刊名称：THERIOGENOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 0093-691X

年卷期： 2023 年 195 卷

页码：

收录情况： SCI

摘要： Cattleyaks are equally adaptable to harsh environment as yaks, but produce far more milk and meat in terms of quality and quantity. However, male cattleyaks with active secondary sexuality are infertile and have restricted productivity and breeding of yaks. Much researches continue to be done in regard to the differences in transcriptome profiling in cattleyak epididymis with respect to yak epididymis. The caput segment of the epididymis is highly specialized for the initiation of spermatozoa maturation, synthesis and secretion. We used RNA-Seq technology to comparatively analyze differentially expressed genes (DEGs) associated with sperm maturation between the caput epididymis of yak and cattleyak. Transcriptomic profiling identified 109 DEGs in which 44 were upregulated and 65 were downregulated. 8 DEGs were validated by quantitative real-time PCR. DEGs were analyzed by GO and KEGG analysis to screen the key genes involved in sperm maturation. The upregulation of PAOX and ATP2C2 may be associated with toxicity and apoptosis resistance in cattleyak with respect to yak. However, downregulated DEFB109, DEFB121, DEFB123, DEFA1, LY6G5C, SLC13A2, CST3, CRYBA4 and ADAM28 were associated with innate immune response, sperm maturation, motility and antimicrobial functions. AMPK and Hedgehog signaling pathways were involved in the top-listed five significantly enriched pathways, and the downregulation of HNF4a and LRP2 may have contributed to infertility in cattleyak. The data provide a powerful resource, contributing to the knowledge on the molecular mechanisms underlying male cattleyak infertility. (c) 2022 Elsevier Inc. All rights reserved.

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