Integrative plasma proteomic and microRNA analysis of Jersey cattle in response to high-altitude hypoxia
文献类型： 外文期刊
作者： Kong, Zhiwei 1 ; Zhou, Chuanshe 1 ; Li, Bin 4 ; Jiao, Jinzhen 1 ; Chen, Liang 1 ; Ren, Ao 1 ; Jie, Hongdong 1 ; Tan, Zhiliang 1 ;
作者机构： 1.Chinese Acad Sci, CAS Key Lab Agroecol Proc Subtrop Reg,Hunan Prov, Natl Engn Lab Pollut Control & Waste Utilizat Liv, South Cent Expt Stn Anim Nutr & Feed Sci,Minist A, Changsha 410125, Hunan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Hunan Coinnovat Ctr Safety Anim Prod CICSAP, Changsha 410128, Hunan, Peoples R China
4.Tibet Autonomous Reg Acad Agr Sci, Inst Anim Husb & Vet, State Key Lab Hulless Barley & Yak Germplasm Reso, Lhasa 850000, Tibet, Peoples R China
5.Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China
关键词： plasma proteomics; microRNA analysis; Jersey cattle; high-altitude hypoxia
期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.225； 五年影响因子：4.987 ）
ISSN： 0022-0302
年卷期： 2019 年 102 卷 5 期
页码：
收录情况： SCI
摘要： Blood has been widely collected and analyzed for diagnosing and monitoring diseases in humans and animals; a range of plasma proteins and peptide can be used as biomarkers to describe pathological or physiological status. Changes in the environment such as high-altitude hypoxia (HAH) can lead to adaptive changes in the blood system of mammals. However, the adaptation mechanism induced by HAH remains unclear. In this study, we used 12 multiparous Jersey cattle (400 +/- 35 kg, average 3 yr old, dry period). We applied an iTRAQ (isobaric tags for relative and absolute quantitation) proteomics approach and microRNA (miRNA) micro-array to explore differences in the plasma proteomic and miRNA profiles of Jersey cattle exposed to HAH conditions in Nyingchi, Tibet (altitude 3,000 m) and HAH-free conditions in Shenyang, China (altitude 50 m). Such quantitative proteomic strategies are suitable for accurate and comprehensive prediction of miRNA targets. In total, 264 differentially expressed proteins (127 upregulated, fold-change > 1.2; 137 downregulated, fold-change < 0.8) and 47 differential miRNAs (25 upregulated, fold-change > 2; 22 downregulated, fold-change < 0.5) were observed in the HAH-stressed group compared with the HAH-free group. Integrative analysis of proteomic and miRNA profiles demonstrated that the biological processes associated with differentially expressed proteins were immune response, complement system, and conjugation system. Integrative analysis of canonical pathways showed that most were associated with acute phase response signaling (z-score = -0.125), liver X receptor/retinoid X receptor (LXR/RXR) activation pathway (z-score = 1.134), coagulation system (z-score = -0.943), and complement system (z-score = -0.632). The current results indicated that Jersey cattle exposed to HAH could adapt to that condition through regulation of inflammatory homeostasis by inhibiting the acute phase response, coagulation system, and complement system and promoting LXR/RXR activation.
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