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The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation

文献类型： 外文期刊

作者： Wei, Yaozong 1 ; Pan, Bo 1 ; Qin, Jianpeng 1 ; Cao, Beijia 1 ; Lv, Tianyi 1 ; Ye, Jiangfeng 1 ; Ning, Ao 1 ; Du, Kunlin 1 ; Chen, Xiangyi 1 ; Zou, Shuqi 1 ; Zang, Shengqin 1 ; Yu, Guozhi 3 ; Song, Tianzeng 4 ; Liang, Qiuxia 3 ; Zhou, Guangbin 1 ;

作者机构： 1.Sichuan Agr Univ, Minist Agr & Rural Affairs, State Key Lab Swine & Poultry Breeding Ind, Key Lab Livestock & Poultry Multi, Chengdu 611130, Peoples R China

2.Sichuan Agr Univ, Coll Anim Sci & Technol, Farm Anim Genet Resources Explorat & Innovat Key L, Chengdu 611130, Peoples R China

3.Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Sichuan, Peoples R China

4.Xizang Acad Agr & Anim Husb Sci, Inst Anim Sci, Lhasa 850009, Xizang, Peoples R China

关键词： Histone lactylation; Oocyte; TW-7; Vitrification; Zygotic genome activation

期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：6.5； 五年影响因子：7.2 ）

ISSN： 1674-9782

年卷期： 2024 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Background Previous studies have shown that the vitrification of metaphase II (MII) oocytes significantly represses their developmental potential. Abnormally increased oxidative stress is the probable factor; however, the underlying mechanism remains unclear. The walnut-derived peptide TW-7 was initially isolated and purified from walnut protein hydrolysate. Accumulating evidences implied that TW-7 was a powerful antioxidant, while its prospective application in oocyte cryopreservation has not been reported.Result Here, we found that parthenogenetic activation (PA) zygotes derived from vitrified MII oocytes showed elevated ROS level and delayed progression of pronucleus formation. Addition of 25 mu mol/L TW-7 in warming, recovery, PA, and embryo culture medium could alleviate oxidative stress in PA zygotes from vitrified mouse MII oocytes, furtherly increase proteins related to histone lactylation such as LDHA, LDHB, and EP300 and finally improve histone lactylation in PA zygotes. The elevated histone lactylation facilitated the expression of minor zygotic genome activation (ZGA) genes and preimplantation embryo development.Conclusions Our findings revealed the mechanism of oxidative stress inducing repressed development of PA embryos from vitrified mouse MII oocytes and found a potent and easy-obtained short peptide that could significantly rescue the decreased developmental potential of vitrified oocytes, which would potentially contribute to reproductive medicine, animal protection, and breeding.

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