Flavonoids from Potentilla anserina protect H9C2 cells from hypoxia/reoxygenation injury by inhibiting mitochondrial apoptosis
文献类型： 外文期刊
作者： Yan, Yingying 1 ; Zhang, Yuhong 1 ; Zhang, Wenhui 1 ; Zhang, Jifeng 2 ;
作者机构： 1.Xizang Acad Agr & Anim Husb Sci XZAAAS, Inst Agriprod Dev & Food Sci IAPDFS, 157 Nongke Rd, Lhasa 850032, Tibet, Peoples R China
2.Xizang Univ, Sch Ecol & Environm, Lhasa, Peoples R China
关键词： Potentilla anserina flavonoids; hypoxia; myocardial cells; oxidative stress; mitochondrial apoptosis
期刊名称：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY （ 影响因子：3.1； 五年影响因子：3.5 ）
ISSN： 0950-5423
年卷期： 2025 年 60 卷 1 期
页码：
收录情况： SCI
摘要： Potentilla anserina L., a valued traditional Chinese medicinal herb and edible plant, has a long history of use for its antioxidant and immunomodulatory properties. Flavonoids extracted from the tuberous roots of P. anserina (silverweed cinquefoil root flavonoids, SCR-F) exhibit protective effects against hypoxia/reoxygenation (H/R) injury in H9C2 cells. In our study, we observed that H/R injury reduces cell viability, increases lactate dehydrogenase release, and elevates levels of reactive oxygen species (ROS) and malondialdehyde (MDA). The protective action of SCR-F on H/R-injured H9C2 cells was concentration-dependent, with efficacy increasing alongside dosage. Notably, the strongest protective effect occurred at a concentration of 208.32 mu g/ml of SCR-F. Treatment with SCR-F significantly improved cell viability decreased ROS and MDA levels, and enhanced the activity of antioxidant enzymes, including catalase, superoxide dismutase, and glutathione peroxidase (GSH-px). Furthermore, SCR-F upregulated the expression of the anti-apoptotic protein Bcl-2, downregulated the pro-apoptotic protein Bax, and decreased caspase-3 and -9 activity. A reduction in voltage-dependent anion channel 1protein coding gene (VDAC)1 expression was also observed, suggesting inhibition of the mitochondrial apoptotic pathway. Our findings indicate that SCR-F protects H9C2 cells from H/R injury by reducing oxidative stress and mitochondrial-mediated apoptosis, underscoring its potential as a therapeutic agent for hypoxia-induced myocardial damage.
- 相关文献
作者其他论文 更多>>
-
Divergent effects of β-glucan addition in baked vs steamed bread: Deciphering hydration of BG and its interaction with starch-protein networks
作者：Zhang, Zhihui;Zhou, Yonghong;Zhang, Zhihui;Pan, Zhifen;Li, Yutong;Zhao, Tianyi;Zhou, Qiang;Bian, Haoquan;Chen, Jiaxin;Wang, Tao;Li, Qiao;Li, Yutong;Zhao, Tianyi;Bian, Haoquan;Zhang, Yuhong;Zeng, Xingquan;Li, Zhongyi
关键词：Hull-less barley; beta-glucan; Water; Starch; Protein; Wheat
-
Refined highland barley ameliorates obesity-associated insulin resistance in high-fat diet-fed mice by targeting the gut microbiota and liver transcriptomics
作者：Wu, Tong;Dai, Zijian;Luo, Yingting;Yu, Qinye;Zhang, Yiyun;Bao, Xin;Li, Rong;Shen, Qun;Xue, Yong;Wu, Tong;Dai, Zijian;Zhang, Yiyun;Bao, Xin;Li, Rong;Shen, Qun;Xue, Yong;Zhang, Yuhong;Hao, Jing;Hao, Jing
关键词：Refined highland barley; Inflammation; Hypoglycemic effect; Gut microbiota; Liver transcriptome
-
Qingke (Highland Barley) from Qinghai-Tibet Plateau: nutritional composition, phenolics profiles, antioxidant activity and hypoglycemic activity
作者：Wu, Yingying;Liu, Yanan;Hou, Dianzhi;Ren, Feiyue;Zhou, Sumei;Wang, Shanshan;Zhang, Yuhong;Zhou, Sumei
关键词：In vitro antioxidant; In vitro hypoglycemia; Monomer phenolics; Phytochemical; Whole grain
-
An update in recent research on nicotine contamination and nicotine-degrading microorganisms
作者：Deng, Mingqin;Basak, Patangal;Song, Jiajia;Suo, Huayi;Deng, Mingqin;Song, Jiajia;Suo, Huayi;Zhang, Yuhong;Suo, Huayi
关键词：Nicotine; Nicotine-degrading microorganisms; Biodegradation; Human health
-
Impact of germination on nutrient substance, antioxidant activity, and phenolic bioaccessibility in highland barley
作者：Liu, Yanan;Wu, Yingying;Jia, Yuanqiang;He, Jialin;Hou, Dianzhi;Ren, Feiyue;Zhou, Sumei;Zhang, Yuhong;Wang, Shanshan;Ren, Feiyue;Zhou, Sumei
关键词：Antioxidant capacity; Bioaccessibility; Germination; Highland barley; Phenolic
-
Effects of different pretreatment methods on starch,non-starch components and production quality of Longzi black highland barley flour under the same gelatinization degree
作者：Liu, Yang;Fu, Liping;Xiao, Hanbing;Zhao, Can;Tian, Yuting;Zhao, Yingting;Zhang, Wenhui;Tian, Yuting;Xiang, Qisen
关键词：Highland barley; Heat treatment; Structural properties; Physicochemical properties
-
A novel strain Lactiplantibacillus plantarum LPP95 isolated from Chinese pickles: Antifungal effect, mechanism, and potential application in yogurt
作者：Wang, Qiang;Zhang, Yu;Wang, Hongwei;Song, Jiajia;Suo, Huayi;Wang, Qiang;Zhang, Yu;Wang, Hongwei;Song, Jiajia;Suo, Huayi;Zhang, Feng;Zhang, Yuhong;Suo, Huayi
关键词：Antifungal activity; Lactiplantibacillus plantarum; Penicillium sp.; Antifungal substance; Yogurt
