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Genome-Wide DNA Methylation and Hydroxymethylation Changes Revealed Epigenetic Regulation of Neuromodulation and Myelination in Yak Hypothalamus

文献类型： 外文期刊

作者： Chai, Zhixin 1 ; Wu, Zhijuan 1 ; Ji, Qiumei 2 ; Wang, Jikun 1 ; Wang, Jiabo 1 ; Wang, Hui 1 ; Zhang, Chengfu 2 ; Zhong, J 1 ;

作者机构： 1.Southwest Minzu Univ, Key Lab Qinghai Tibetan Plateau Anim Genet Resour, Sichuan Prov & Minist Educ, Chengdu, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, State Key Lab Hulless Barley & Yak Germplasm Reso, Inst Anim Sci & Vet Res, Lhasa, Peoples R China

关键词： DNA methylation; hydroxymethylation; neuromodulation; myelination; hypothalamus

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN：

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are important epigenetic modifications in neurodevelopment. However, there is little research examining the genome-wide patterns of 5mC and 5hmC in brain regions of animals under natural high-altitude conditions. We used oxidative reduced representation bisulfite sequencing (oxRRBS) to determine the 5mC and 5hmC sites in the brain, brainstem, cerebellum, and hypothalamus of yak and cattle. We reported the first map of genome-wide DNA methylation and hydroxymethylation in the brain, brainstem, cerebellum, and hypothalamus of yak (living at high altitudes) and cattle. Overall, we found striking differences in 5mC and 5hmC between the hypothalamus and other brain regions in both yak and cattle. Genome-wide profiling revealed that 5mC level decreased and 5hmC level increased in the hypothalamus than in other regions. Furthermore, we identified differentially methylated regions (DMRs) and differentially hydroxymethylated regions (DhMRs), most of which overlapped with each other. Interestingly, transcriptome results for these brain regions also showed distinctive gene levels in the hypothalamus. Finally, differentially expressed genes (DEGs) regulated by DMRs and DhMRs may play important roles in neuromodulation and myelination. Overall, our results suggested that mediation of 5mC and 5hmC on epigenetic regulation may broadly impact the development of hypothalamus and its biological functions.

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