文献类型： 外文期刊

作者： Cao, Yongqing ¹ ; Zeng, Tao ² ; Han, Wei ³ ; Ma, Xueying ⁵ ; Gu, Tiantian ² ; Chen, Li ² ; Tian, Yong ² ; Xu, Wenwu ² ; Yin, Jianmei ³ ; Li, Guohui ³ ; Lu, Lizhi ² ; Gun, Shuangbao ¹ ;

作者机构： 1.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou 730070, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

3.Jiangsu Inst Poultry Sci, Yangzhou 225125, Peoples R China

4.Technol Innovat Co Ltd, Jiangsu Inst Poultry Sci, Yangzhou 211412, Peoples R China

5.Tibet Acad Agr & Anim Husb Sci, Inst Anim Husb & Vet Med, Lhasa 850004, Peoples R China

6.China Ukraine Joint Res Ctr Protect Exploitat & Ut, Hangzhou 310021, Peoples R China

关键词： Hypoxia Adaptation; Lipid Metabolism; Liver; Tibetan Chicken; Transcriptome

期刊名称：ANIMAL BIOSCIENCE （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 2765-0189

年卷期： 2024 年 37 卷 1 期

页码：

收录情况： SCI

摘要： Objective: Tibetan chickens, which have unique adaptations to extreme high -altitude environments, exhibit phenotypic and physiological characteristics that are distinct from those of lowland chickens. However, the mechanisms underlying hypoxic adaptation in the liver of chickens remain unknown. Methods: RNA -sequencing (RNA-Seq) technology was used to assess the differentially expressed genes (DEGs) involved in hypoxia adaptation in highland chickens (native Tibetan chicken [HT]) and lowland chickens (Langshan chicken [LS], Beijing You chicken [BJ], Qingyuan Partridge chicken [QY], and Chahua chicken [CH]). Results: A total of 352 co-DEGs were specifically screened between HT and four native lowland chicken breeds. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analyses indicated that these co-DEGs were widely involved in lipid metabolism processes, such as the peroxisome proliferator-activated receptors (PPAR) signaling pathway, fatty acid degradation, fatty acid metabolism and fatty acid biosynthesis. To further determine the relationship from the 352 co-DEGs, protein -protein interaction network was carried out and identified eight genes (ACSL1, CPT1A, ACOX1, PPARC1A, SCD, ACSBG2, ACACA, and FASN) as the potential regulating genes that are responsible for the altitude difference between the HT and other four lowland chicken breeds. Conclusion: This study provides novel insights into the molecular mechanisms regulating hypoxia adaptation via lipid metabolism in Tibetan chickens and other highland animals.