文献类型： 外文期刊

作者： Wang, Xinjian ¹ ; Yang, Zhen ¹ ; Liu, Yu ¹ ; Wang, Xuehong ¹ ; Zhang, Hongjuan ¹ ; Shang, Ruofeng ¹ ; Laba, Cidan ² ; Wujin, Cuomu ² ; Hao, Baocheng ¹ ; Wang, Shengyi ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab New Anim Drug Project, Key Lab Vet Pharmaceut Dev,Minist Agr & Rural Affa, Lanzhou 730050, Gansu, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Inst Anim Sci & Vet, Lhasa 850009, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.6； 五年影响因子：4.9 ）

ISSN： 2045-2322

年卷期： 2022 年 12 卷 1 期

页码：

收录情况： SCI

摘要： In this paper, Nostoc commune crude polysaccharide was extracted by heating and Ultrasonic-assisted methods separately, homogeneous polysaccharide HNCP3 and UNCP4 were obtained after purified by DEAE-52 cellulose column chromatography and Sephacryl G-100 gel column chromatography. The structures of HNCP3 and UNCP4 were characterized by molecular weight determination, infrared spectroscopy, DSC detection, sodium periodate oxidation, smith degradation reaction and methylation analysis. The conformation of the solution was studied by SEM and AFM. The results showed that the Ultrasonic-assisted extraction had effects on the molecular weight, monosaccharide composition, molar ratio and configuration of Nostoc commune. The main chain of HNCP3 and UNCP4 was & RARR; 6)-D-Glcp(1 & RARR; and & RARR; 2, 6)-D-Glcp, but UNCP4 contained 1, 2, 6-galactose and 2, 3-Me2-D-Ara branches, while HNCP3 did not. The results of the monosaccharides composition of indicated that mannose was presented in both HNCP3 and UNCP4. SEM and AFM showed that the structure of UNCP4 was helical, and the solution conformations of HNCP3 and UNCP4 were different in different solution environments. Studies on DPPH radicals, superoxide anions, and hydroxyl radicals scavenging abilities showed that UNCP4 had higher antioxidant activity, while studies on the antidiabetic activities showed that the hypoglycemic effect of UNCP4 was stronger than that of HNCP3. Therefore, Ultrasonic-assisted extraction (UAE) increases the bioactivity of Nostoc commune polysaccharide (NCP) as well as the extraction rate.