Cryosurvival and pregnancy rates: One-step protocol for freezing-thawing Shangri-la Yak (Bos grunniens) Embryos
文献类型： 外文期刊
作者： Jia, Yinhai 1 ; Yang, Xiurong 1 ; Zhang, Chengfu 3 ; Yang, Shangzhi 4 ; Li, Ming 5 ; Xu, Wenwen 1 ; Ji, Qiumei 3 ; Jiang, 1 ;
作者机构： 1.Guangxi Univ, Coll Anim Sci & Technol, Nanning, Peoples R China
2.Guangxi Univ, Coll Anim Sci & Technol, Nanning 530005, Peoples R China
3.Tibet Acad Agr & Anim Husb Sci, Inst Husb & Pharmaceut Sci, Lhasa, Peoples R China
4.Shangri La Zanglong Biol Dev Co Ltd, Di Qing, Peoples R China
5.Guangxi Autonomous Reg Livestock Breed Improvemen, Nanning, Peoples R China
关键词： cryosurvival; freezing-thawing; one-step protocol; Shangri-la yak
期刊名称：REPRODUCTION IN DOMESTIC ANIMALS （ 影响因子：2.005； 五年影响因子：2.154 ）
ISSN： 0936-6768
年卷期： 2018 年 53 卷 5 期
页码：
收录情况： SCI
摘要： The yak is one of the most important and economically useful animals for highlanders. The decline in the yak population requires effective measures for the conservation and multiplication of elite germplasm. A standardized protocol will simplify the freezing and warming of yak embryos in straw and facilitate embryo transfer. In this work, we investigated a one-step protocol that uses a stable basal medium, which comprised a warming medium (1.08M sucrose) and a freezing medium (EFS40). We also assessed the effects of the new transfer method on embryo survival. A total of 145 yak frozen embryos were thawed in a standard medium system. The one-step protocol led to a high recovery percentage (84.93) of yak embryos that survived vitrification and warming. The in vitro survival rates of these embryos significantly different from those of embryos frozen-thawed via the conventional method. The 95 embryos frozen-thawed via our one-step protocol were then implanted in selected recipients. Thirty-six singleton pregnancies were established. In conclusion, the proposed one-step method is a simple, safe, and standardized freezing-thawing protocol that ensures embryo survival and quality under field conditions. This study establishes new possibilities for the widespread use of embryo transfer in yaks.
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