Novel mechanism of Clostridium butyricum alleviated coprophagy prevention-induced intestinal inflammation in rabbit
文献类型： 外文期刊
作者： Li, Zhichao 1 ; He, Hui 1 ; Chen, Mengjuan 1 ; Ni, Mengke 1 ; Guo, Chaohui 1 ; Wan, Zhiyi 3 ; Zhou, Jianshe 4 ; Wang, Zhitong 1 ; Wang, Yaling 1 ; Cai, Hanfang 1 ; Li, Ming 1 ; Sun, HuiZeng 2 ; Xu, Huifen 1 ;
作者机构： 1.Henan Agr Univ, Coll Anim Sci & Technol, Zhengzhou 450046, Peoples R China
2.Zhejiang Univ, Inst Dairy Sci, Coll Anim Sci, Hangzhou 310058, Peoples R China
3.China Agr Univ, Coll Biol Sci, 2 Yuan Ming Yuan West Rd, Beijing 100193, Peoples R China
4.Tibet Acad Agr & Anim Husb Sci, Inst Fisheries Sci, Lhasa 850032, Peoples R China
关键词： Coprophagy prevention; Inflammation; Microbiome; Rabbits; Clostridium butyricum; TLR5
期刊名称：INTERNATIONAL IMMUNOPHARMACOLOGY （ 影响因子：5.6； 五年影响因子：5.6 ）
ISSN： 1567-5769
年卷期： 2024 年 130 卷
页码：
收录情况： SCI
摘要： As bacteria synthesize nutrients primarily in the cecum, coprophagy is indispensable for supplying rabbits with essential nutrients. Recent research has demonstrated its pivotal role in maintaining intestinal microbiota homeostasis and immune regulation in rabbits, although the specific mechanism remains unknown. Here, we used coprophagy prevention (CP) to investigate the effects of coprophagy on the cecum homeostasis and microbiota in New Zealand white rabbits. Furthermore, whether supplementation of Clostridium butyricum (C. butyricum) may alleviate the cecum inflammation and apoptosis caused by CP was also explored. Four groups were randomly assigned: control (Con), sham-coprophagy prevention (SCP), coprophagy prevention (CP), and CP and C. butyricum addition (CPCB). Compared to Con and SCP, CP augmented cecum inflammation and apoptosis, as well as bacterial adhesion to the cecal epithelial mucosa, while decreasing the expression of tight junction proteins (ZO-1, occluding, and claudin-1). The relative abundance of short-chain fatty acids (SCFAs)-producing bacteria was significantly decreased in the CP group. Inversely, there was an increase in the Firmicutes/Bacteroidetes ratio and the relative abundance of Christensenellaceae_R-7_group. Additionally, CP increased the levels of Flagellin, IFN-gamma, TNF-a, and IL-1 beta in cecum contents and promoted the expression of TLR5/MyD88/NF-kappa B pathway in cecum tissues. However, the CPCB group showed significant improvements in all parameters compared to the CP group. Dietary C. butyricum supplementation significantly increased the production of SCFAs, particularly butyric acid, triggering anti-inflammatory, tissue repairing, and barrier-protective responses. Notably, CPCB effectively mitigated CP-induced apoptosis and inflammation. In summary, CP disrupts the cecum epithelial barrier and induces inflammation in New Zealand white rabbits, but these effects can be alleviated by C. butyricum supplementation. This process appears to be largely associated with the TLR5/MyD88/NF-kappa B signaling pathway.
- 相关文献
作者其他论文 更多>>
-
Single-cell transcriptome analysis reveals a cellular immune response in brown trout ( Salmon trutta fario) ) infected with Aeromonas salmonicida
作者：Sun, Shuaijie;Lei, Kuankuan;Wang, Wanliang;Wang, Zhuangzhuang;Zhou, Jianshe;Li, Zhichao;Li, Zhichao;Lei, Kuankuan;Sun, Minshan;Sun, Shuaijie;Wang, Wanliang;Wang, Zhuangzhuang;Zhou, Jianshe;Wang, Wanliang;Wang, Zhuangzhuang;Zhou, Jianshe
关键词：Single-cell transcriptome; Brown trout; Aeromonas salmonicida; Immune response; Resistance response
-
Transcriptome analysis of glyptosternum maculatum (sisoridae: siluriformes) provided insights into sexual dimorphism, Xizang, China
作者：He, Haoming;Wu, Mengyu;Wang, Zhuangzhuang;Zhou, Jianshe;Wang, Wanliang;He, Haoming;Wu, Mengyu;Wang, Zhuangzhuang;Zhou, Jianshe;Wang, Wanliang
关键词：
Glyptosternum maculatum ; RNA-sequencing; Illumina; Sex-determination; Sex difference gene; Gene interaction -
Multi-Omics Analysis Reveals the Regulatory Mechanism of Different Probiotics on Growth Performance and Intestinal Health of Salmo trutta (S. trutta)
作者：Chen, Mengjuan;Wang, Zhitong;He, Hui;He, Wenjia;Zhang, Zihao;Sun, Shuaijie;Wang, Wanliang;Chen, Mengjuan;Wang, Zhitong;He, Hui;He, Wenjia;Zhang, Zihao;Sun, Shuaijie;Wang, Wanliang;Wang, Wanliang
关键词：Salmo trutta; probiotics; gut microbiome; gut metabolome; intestinal function
-
Multi-omic Analyses Shed Light on The Genetic Control of High-altitude Adaptation in Sheep
作者：Li, Chao;Chen, Bingchun;Zhou, Shiwei;Kalds, Peter;Zhang, Ke;Huang, Shuhong;Li, Ran;Li, Yan;Fang, Wenwen;Cai, Yudong;Jiang, Yu;Chen, Yulin;Wang, Xiaolong;Li, Chao;Bhati, Meenu;Leonard, Alexander;Pausch, Hubert;Langda, Suo;Cuoji, Awang;Wu, Yujiang;Cuomu, Renqin;Gui, Ba;Pu, Peng;Zhu, Xiaojia;Wang, Xiran;Zhu, Haolin;Li, Ming;Wang, Yutao;Jia, Ting;Pu, Tianchun;Pan, Xiangyu;He, Chong;Zhou, Ping;Zhou, Ping;Han, Jian-Lin;Han, Jian-Lin
关键词：Environmental adaptation; High altitude; Hypoxia; Selection signature; Sheep
-
Correlation Analysis of the Transcriptome and Gut Microbiota in Salmo trutta Resistance to Aeromonas salmonicida
作者：Sun, Shuaijie;Lei, Kuankuan;Wang, Zhuangzhuang;Wang, Wanliang;Zhou, Jianshe;Sun, Shuaijie;Lei, Kuankuan;Li, Zhichao;Li, Ming;Sun, Shuaijie;Lv, Jun
关键词：
Salmo trutta ;Aeromonas salmonicida ; RNA-seq; 16S rRNA; resistance -
Genome-wide identification of simple sequence repeats and development of polymorphic SSR markers in brown trout (Salmo trutta)
作者：Ma, Haibing;Huang, Tianqing;Gu, Wei;Liu, Enhui;Wang, Gaochao;Wang, Bingqian;Xu, Gefeng;Zhou, Jianshe
关键词：Brown trout (Salmo trutta); Microsatellite markers; Population genetics; Bioinformatics; Salmon; Genetic resource conservation
-
Liver transcriptome analysis revealing response to high-temperature stress in Glyptosternum maculatum (Sisoridae: Siluriformes)
作者：Liu, Haiping;Wang, Wanliang;Zhou, Jianshe;He, Hui;Sun, Shuaijie;Zhang, Bianbian
关键词：Glyptosternum maculatum; Transcriptome analysis; Liver; Temperature stress
