Structural and functional characterization of peptide YY on feeding in Schizothorax davidi
文献类型： 外文期刊
作者： Zhou, Chaowei 1 ; Lei, Luo 1 ; Yuan, Dengyue 2 ; Deng, Xingxing 1 ; Ye, Hua 1 ; Luo, Hui 1 ; Fang, Jiayang 1 ; Yang, Minm 1 ;
作者机构： 1.Southwest Univ, Coll Anim Sci, Chongqing 402460, Peoples R China
2.Neijiang Normal Univ, Dept Aquaculture, Coll Life Sci, Neijiang, Sichuan, Peoples R China
3.Tibet Acad Agr & Anim Husb Sci, Lhasa, Tibet, Peoples R China
4.Tibet Acad Agr & A
关键词： cloning; fasting; feeding; PYY; Schizothorax davidi
期刊名称：JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY （ 影响因子：2.553； 五年影响因子：2.019 ）
ISSN： 2471-5646
年卷期： 2018 年 329 卷 2 期
页码：
收录情况： SCI
摘要： Several studies have demonstrated that the neuropeptide peptide YY (PYY) plays an important role in feeding in mammals and fish. However, thus far, the feeding regulation function of PYY in Schizothorax davidi has not been well understood. Here, we identified the full-length cDNA sequence of PYY in S. davidi for the first time. S. davidi PYY contains 803bp nucleotides including a 328bp 3 untranslated region (UTR), a 181bp 5 UTR, and a 294bp open reading frame encoding a peptide of 97 amino acids. S. davidi PYY expression was observed in almost all tissues, with the highest expression detected in the hypothalamus. PYY mRNA expression in the hypothalamus was significantly elevated after a meal (P<0.01), and significantly decreased after fasting (P<0.01). PYY expression levels were increased sharply following refeeding after 9 days (P<0.01), suggesting that it might function as a satiety factor in S. davidi.
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