Halomonas sp. NEC-1, an ectoine-rich and wide pH tolerant halophilic bacterium isolated from a salt lake of Xizang, China
文献类型： 外文期刊
作者： Ma, Yingchao 1 ; Sun, Zhongshi 1 ; Li, Yukang 1 ; Zou, Hanying 1 ; Zhao, Yuanliang 1 ; Lin, Feiyang 1 ; Han, Xuekai 1 ; Sui, Liying 1 ; Zhang, Chi 2 ;
作者机构： 1.Tianjin Univ Sci & Technol, Coll Marine & Environm Sci, Asian Reg Artemia Reference Ctr, Tianjin 300457, Peoples R China
2.Xizang Acad Agr & Anim Husb Sci, Inst Fisheries Sci, Lhasa 850032, Peoples R China
关键词： halophilic microorganism; ectoine; salt lake; Xizang
期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.3； 五年影响因子：1.5 ）
ISSN： 2096-5508
年卷期： 2025 年 43 卷 5 期
页码：
收录情况： SCI
摘要：
A halophilic bacterium, named Halomonas sp. NEC-1 was isolated from the Nyer Co Salt Lake on the Xizang Plateau, SW China. The strain exhibited a broad pH tolerance range of 5-11, distinguishing it from Halomonas alkaliphila despite a 99% genetic similarity. Optimal growth conditions, determined through orthogonal experiments, were found to be 37 degrees C, 100-g/L salinity, and an initial pH of 6, resulting in a maximum OD600 of 7.98 +/- 0.06. Halomonas sp. NEC-1 produced 545.43 +/- 25.10 mg/L of ectoine under optimal conditions of 75-g/L salinity, 40-g/L sodium glutamate, and an initial pH of 6. This production increased to 1 388.81 +/- 3.69 mg/L after five rounds of hypo-osmotic shocks. During the shocks, ectoine productivity remained stable at approximately 16.29 +/- 0.04 to 17.28 +/- 0.48 mg/(L
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