Comparative proteomics analysis of Tibetan hull-less barley under osmotic stress via data-independent acquisition mass spectrometry
文献类型： 外文期刊
作者： Wang, Yulin 1 ; Sang, Zha 1 ; Xu, Shaohang 3 ; Xu, Qijun 1 ; Zeng, Xingquan 1 ; Jabu, Dunzhu 1 ; Yuan, Hongjun 1 ;
作者机构： 1.State Key Lab Hulless Barley & Yak Germplasm Reso, 130 Jinzhu West Rd, Lhasa 850002, Tibet, Peoples R China
2.Tibet Acad Agr & Anim Husb Sci, Inst Agr Res, 130 Jinzhu West Rd, Lhasa 850002, Tibet, Peoples R China
3.Deepxomics Co Ltd, 2082 Shenyan Rd, Shenzhen 518000, Guangdong, Peoples R China
关键词： Tibetan hull-less barley; osmotic stress; proteomics; DIA; quantification; abiotic stress
期刊名称：GIGASCIENCE （ 影响因子：6.524； 五年影响因子：8.702 ）
ISSN： 2047-217X
年卷期： 2020 年 9 卷 3 期
页码：
收录情况： SCI
摘要： Background: Tibetan hull-less barley (Hordeum vulgare L. var. nudum) is one of the primary crops cultivated in the mountains of Tibet and encounters low temperature, high salinity, and drought. Specifically, drought is one of the major abiotic stresses that affect and limit Tibetan barley growth. Osmotic stress is often simultaneously accompanied by drought conditions. Thus, to improve crop yield, it is critical to explore the molecular mechanism governing the responses of hull-less barley to osmotic/drought stress conditions. Findings: In this study, we used quantitative proteomics by data-independent acquisition mass spectrometry to investigate protein abundance changes in tolerant (XL) and sensitive (DQ) cultivars. A total of 6,921 proteins were identified and quantified in all samples. Two distinct strategies based on pairwise and time-course comparisons were utilized in the comprehensive analysis of differentially abundant proteins. Further functional analysis of differentially abundant proteins revealed that some hormone metabolism-associated and phytohormone abscisic acid-induced genes are primarily affected by osmotic stress. Enhanced regulation of reactive oxygen species (may promote the tolerance of hull-less barley under osmotic stress. Moreover, we found that some regulators, such as GRF, PR10, MAPK, and AMPK, were centrally positioned in the gene regulatory network, suggesting that they may have a dominant role in the osmotic stress response of Tibetan barley. Conclusions: Our findings highlight a subset of proteins and processes that are involved in the alleviation of osmotic stress. In addition, this study provides a large-scale and multidimensional proteomic data resource for the further investigation and improvement of osmotic/drought stress tolerance in hull-less barley or other plant species.
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