Integrated Transcriptomics and Metabolomics Analyses Provide Insights into Qingke in Response to Cold Stress
文献类型： 外文期刊
作者： Xu, Congping 1 ; Gui, Zihao 1 ; Huang, Yuxiao 4 ; Yang, Haizhen 2 ; Luo, Jie 4 ; Zeng, Xingquan 2 ;
作者机构： 1.Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan 430023, Peoples R China
2.Minist Agr, Key Lab Barley Biol & Genet Improvement QingHai Ti, Lhasa 850002, Peoples R China
3.Tibet Acad Agr & Anim Husb Sci, Lhasa 850002, Peoples R China
4.Hainan Yazhou Bay Grain Lab, Sanya 572025, Peoples R China
关键词： cold stress; lipid metabolism; GDSL; CBF; transcriptome; metabolome
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2023 年 71 卷 47 期
页码：
收录情况： SCI
摘要： The survival and productivity of qingke in high altitude (>4300 m, average yearly temperature <0 C-degrees) of the Tibetan Plateau are significantly impacted by low-temperature stress. Uncovering the mechanisms underlying low-temperature stress response in cold-tolerant qingke varieties is crucial for qingke breeding. Herein, we conducted a comprehensive transcriptomic and metabolomic analysis on cold-sensitive (ZQ) and cold-tolerant (XL) qingke varieties under chilling and freezing treatments and identified lipid metabolism pathways as enriched in response to freezing treatment. Additionally, a significant positive correlation was observed between the expression of C-repeat (CRT) binding factor 10A (HvCBF10A) and Gly-Asp-Ser-Leu-motif lipase (HvGDSL) and the accumulation of multiple lipids. Functional analysis confirmed that HvCBF10A directly binds to HvGDSL, and silencing HvCBF10A resulted in a significant decrease in both HvGDSL and lipid levels, consequently impairing the cold tolerance. Overall, HvCBF10A and HvGDSL are functional units in actively regulating lipid metabolism to enhance freezing stress tolerance in qingke.
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