文献类型： 外文期刊

作者： Song, Tianzeng ¹ ; Shi, Yi ¹ ; Wang, Yangang ¹ ; Qazi, Izhar Hyder ¹ ; Angel, Christiana ⁴ ; Zhang, Ming ¹ ;

作者机构： 1.Sichuan Agr Univ, Coll Anim Sci & Technol, Chengdu 611130, Sichuan, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Inst Anim Sci, Lhasa 850009, Peoples R China

3.Shaheed Benazir Bhutto Univ Vet & Anim Sci, Dept Vet Anat & Histol, Sakrand 67210, Pakistan

4.Sichuan Agr Univ, Coll Vet Med, Dept Vet Parasitol, Chengdu 611130, Sichuan, Peoples R China

5.Shaheed Benazir Bhutto Univ Vet & Anim Sci, Fac Vet Sci, Dept Vet Parasitol, Sakrand 67210, Pakistan

关键词： apoptosis; boar; lipopolysaccharide; polyhistidine (pHis); sperm

期刊名称：ANIMALS （ 影响因子：2.752； 五年影响因子：2.942 ）

ISSN： 2076-2615

年卷期： 2019 年 9 卷 10 期

页码：

收录情况： SCI

摘要： Simple Summary Gram-negative bacteria are the main pathogenic microorganisms found in human and animal semen. Lipopolysaccharide (LPS), a component of the cell wall of Gram-negative bacteria, has been linked to inducing apoptosis in human and rat sperm; however, little is known regarding LPS-induced apoptosis in boar sperm. This detrimental effect of LPS is potentially mediated via competitive bidding with toll-like receptor (TLR) 4 on the cytoplasmic membrane. Therefore, it is reasonable to elucidate the potential mechanisms by which the binding of LPS and TLR4 could be prevented. Polyhistidine is widely used for the delivery of nucleic acids and antibodies into the cell cytoplasm, and it is a novel TLR4 agonist. In the current study, we envisaged that pHis might also serve as an effective tool for inhibiting LPS-induced apoptosis in boar sperm. The new finding of our present study is that pHis could inhibit, to some extent, LPS-induced boar sperm apoptosis, and it could ameliorate the overall sperm quality parameters under liquid storage or at 37 degrees C incubation conditions. However, further investigation should be continued to fully elucidate the mechanistic basis of these ameliorative effects of pHis. Lipopolysaccharide (LPS) released from Gram-negative bacteria binds to toll-like receptor 4 (TLR4) and induces boar sperm apoptosis. Similarly, polyhistidine (pHis), a TLR4 agonist, can also bind to TLR4. We hypothesized that pHis could inhibit LPS-induced sperm apoptosis by competitively binding to TLR4 to then improve sperm quality. Therefore, the objective of this study was to examine whether pHis can inhibit LPS-induced sperm apoptosis and affect sperm quality. The results showed that the concentrations of bacterial colonies were significantly increased from 36 to 120 h under liquid storage conditions (p < 0.05); however, concentrations of LPS in boar semen showed a relatively constant trend (4.98 +/- 1.55 EU/mL) following 120 h storage. The addition of 100 mu g/mL pHis in the BTS extender significantly improved boar sperm motility and viability at 37 degrees C, and it significantly (p < 0.05) inhibited boar sperm apoptosis under liquid storage (17 degrees C) and at 37 degrees C incubation conditions. The co-treatment of LPS and pHis further confirmed that pHis played its role in inhibiting LPS-induced sperm apoptosis. In conclusion, our preliminary findings provide reasonable evidence that pHis could act as an inhibitor of LPS-induced apoptosis in boar sperm stored for longer periods of time. pHis might inhibit LPS-induced sperm apoptosis by competitively binding to TLR4. Nevertheless, further mechanistic studies are awaited to fully elucidate its potential implication in inhibiting LSP-induced apoptosis.