Prevalence and genetic characterization of Enterocytozoon bieneusi and Giardia duodenalis in Tibetan pigs in Tibet, China
文献类型： 外文期刊
作者： Zou, Yang 1 ; Zheng, Wen-Bin 1 ; Song, Hai-Yang 1 ; Xia, Chen-Yang 3 ; Shi, Bin 3 ; Liu, Jian-Zhi 3 ; Hou, Jun-Ling 1 ; Z 1 ;
作者机构： 1.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China
2.Hunan Agr Univ, Coll Vet Med, Hunan Prov Key Lab Prot Engn Anim Vaccines, Changsha 410128, Hunan, Peoples R China
3.Tibet Acad Agr & Anim Husb Sci, Inst Livestock Res, Lhasa 850009, Tibet Autonomou, Peoples R China
4.Yangzhou Univ, Coll Vet Med, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
关键词： Enterocytozoon bieneusi; Giardia duodenalis; Prevalence; Genotypes; Multilocus sequence typing; Tibetan pigs; China
期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：3.342； 五年影响因子：3.188 ）
ISSN： 1567-1348
年卷期： 2019 年 75 卷
页码：
收录情况： SCI
摘要： Enterocytozoon bieneusi and Giardia duodenalis are important opportunistic enteric zoonotic pathogens that cause diarrhoea and intestinal diseases in animals and humans. China is the largest producer of pigs, but whether Tibetan pigs, a unique pig breed in Tibet, are infected with E. bieneusi and G. duodenalis is unknown. Therefore, we conducted a molecular epidemiological survey to determine the prevalence of E. bieneusi and G. duodenalis in Tibetan pigs in Tibet, China, and identified the genotypes of these causative agents. A total of 345 faecal specimens were collected from Tibetan pigs from three Tibet counties (Milin, Cuona and Gongbujiangda), examined by nested PCR and sequenced utilizing genetic markers in the ribosomal internal transcribed spacer (ITS) region of the rRNA and glutamate dehydrogenase (gdh) gene for E. bieneusi and G. duodenalis, respectively. Moreover, using multilocus sequence typing, the subtypes of E. bieneusi were identified based on four loci (MS1, MS3, MS4 and MS7). A total of 41 (11.88%) faecal samples from Tibetan pigs were E. bieneusi-positive, and 2 (0.58%) were G. duodenalis-positive. The multivariate logistic regression analysis showed that age was considered a risk factor for Tibetan pig infection of E. bieneusi. Two novel (GB11, GB31) and four known E. bieneusi genotypes (EbpC, EbpD, PigEBITS5 and CHS12) were identified and were all classified as zoonotic group 1 according to the phylogenetic analysis. Two MLGs (MLGI and MLGII) were further identified in the E. bieneusi EbpC genotype by multilocus sequence typing analysis. In addition, two G. duodenalis assemblages (D and E) were found in the present study. To our knowledge, the current study is the first to detect the prevalence and perform genetic characterization of G. duodenalis in Tibetan pigs in Tibet, China. The results could provide essential data for controlling E. bieneusi and G. duodenalis infections in Tibetan pigs that are in contact with other animals and humans, as Tibetan pigs could be a potential source for human infection by these pathogens.
- 相关文献
作者其他论文 更多>>
-
Morphological and Mitochondrial Genome Characterization of Hypoderma Sinense: Insights into Evolutionary Relationships Within Oestridae
作者：Shi, Bin;Dong, Hai-Long;Wu, Qing-Xia;Shi, Bin;Zhao, Xia-Ling;Tang, Wen-Qiang;Gan, Fu-Bin;Qi, Ming;Li, Xiao-Ya;Li, Yan-Bin;Huang, Fu-Qiang;Zhang, Ming-Zhu;Ali, Munwar;Li, Kun;El-Mansi, Ahmed A.;Gadallah, Abdelalim A.
关键词：Hypoderma sinense; Mitochondrial genome; Oestridae; Phylogenetic relationships
-
Global research landscape and trends in Toxoplasma gondii from 2003 to 2022: A bibliometric analysis
作者：Fan, Shi-Jie;Pan, Ming;Huang, Si-Yang;Fan, Shi-Jie;Pan, Ming;Huang, Si-Yang;Fan, Shi-Jie;Pan, Ming;Huang, Si-Yang;Xia, Chen-Yang;Yang, Pin;Huang, Si-Yang
关键词：T. gondii; Bibliometrics; Hotpot; Scopus; VOSviewer; CiteSpace; Scimago Graphica
-
Prevalence and Molecular Characterization of Parasitic Lice in Tibetan Yaks, Pigs and Sheep
作者：Luo, Wanmei;Wu, Qingxia;Zhao, Xialing;Shi, Bin;Tang, Wenqiang;Wang, Dengyun;Nawaz, Shah
关键词：ectoparasites; lice infestation; phylogenetic analysis; rrnS gene; Haematopinus apri
-
Characterization and enzymatic function of thioredoxin glutathione reductase in Orientobilharzia turkestanicum isolated from Xizang
作者：Tang, Wenqiang;Shi, Bin;Zhao, Xialing;Yue, Yongcheng;Hong, Yang;Hong, Yang
关键词：Thioredoxin glutathione reductase (TGR); Thioredoxin glutathione reductase with selenocysteine (TGRsec); RNA interference; Vaccine; Orientobilharzia turkestanicum; Thioredoxin glutathione reductase (TGR); Thioredoxin glutathione reductase with selenocysteine (TGRsec); RNA interference; Vaccine
-
Prevalence and relative-risks of pasteurella in yaks of Xizang, China
作者：Ma, Hongcai;Wang, Dongjing;Yuan, Zhenjie;Shi, Bin;Zeng, Jiangyong;Meng, Fanxing;Zhao, Congming;Pan, Chang
关键词：ELISA;
Pasteurella ; Risk factor analysis; Seroepidemiology; Yak -
Phylogenetic analysis and biological characteristics of an Akabane virus isolated in China
作者：Li, Gan;Cai, Shikai;Liu, Xiyu;Li, Hairui;Qin, Limei;Zhao, Dengshuai;Huang, Junjie;Li, Ping;Zhang, Yuanhang;Zheng, Yajie;Zhang, Miaomiao;Gao, Han;Zhao, Mengmeng;Zhang, Keshan;Tang, Wenqiang;Zhao, Xialing;Shi, Bin;Qi, Wanxiang
关键词：Akabane virus; genetic variation; phylogeny; antigenic epitope; pathogenicity
-
Integrating network pharmacology, molecular docking, and molecular dynamics simulations to explore potential compounds and mechanisms of Coptis chinensis in treating streptococcal infections
作者：Qi, Wanxiang;Shi, Bin;Tang, Wenqiang;Zeng, Jiangyong;Zhuo, Ma;Ma, Hongcai
关键词：Streptococcus; Coptis chinensis; Network pharmacology; Molecular docking; Molecular dynamics
