Characterization and enzymatic function of thioredoxin glutathione reductase in Orientobilharzia turkestanicum isolated from Xizang
文献类型： 外文期刊
作者： Tang, Wenqiang 1 ; Yue, Yongcheng 2 ; Shi, Bin 1 ; Zhao, Xialing 1 ; Hong, Yang 3 ;
作者机构： 1.Tibet Acad Agr & Anim Husb Sci, Inst Anim Sci, Lhasa 850000, Peoples R China
2.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Natl Reference Lab Anim Schistosomiasis, Key Lab Anim Parasitol,Minist Agr & Rural Affairs, Shanghai 200241, Peoples R China
3.Chinese Ctr Dis Control & Prevent, Chinese Ctr Trop Dis Res,Natl Hlth Commiss Peoples, Natl Inst Parasit Dis,WHO,Collaborating Ctr Trop, Key Lab Parasite & Vector Biol,Natl Ctr Int Res Tr, Shanghai 200025, Peoples R China
4.Hainan Trop Dis Res Ctr, Haikou 571199, Peoples R China
关键词： Thioredoxin glutathione reductase (TGR); Thioredoxin glutathione reductase with selenocysteine (TGRsec); RNA interference; Vaccine; Orientobilharzia turkestanicum; Thioredoxin glutathione reductase (TGR); Thioredoxin glutathione reductase with selenocysteine (TGRsec); RNA interference; Vaccine
期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.6； 五年影响因子：2.7 ）
ISSN：
年卷期： 2025 年 21 卷 1 期
页码：
收录情况： SCI
摘要： Orientobilharziasis, caused by Orientobilharzia turkestanicum, is a zoonotic parasitic disease that leads to significant economic losses in livestock and cercarial dermatitis in humans. This study focuses on the molecular characterization and functional analysis of thioredoxin glutathione reductase (TGR) from O. turkestanicum, a key enzyme involved in the parasite's antioxidant defense system. The full-length O. turkestanicum thioredoxin glutathione reductase (OtTGR) cDNA and O. turkestanicum thioredoxin glutathione reductase with selenocysteine (OtTGRsec) were cloned and expressed as recombinant proteins in E. coli. Western blotting confirmed the specific immunoreactivity of rOtTGR with polyclonal antibodies, and immunohistochemistry revealed its predominant localization on the tegument of adult worms. Enzymatic activity assays demonstrated that rOtTGRsec possesses thioredoxin reductase, glutaredoxin, and glutathione reductase activities, with optimal activity under physiological pH and temperature conditions. RNA interference assays showed that siRNA3 effectively suppressed OtTGR expression in vitro, reducing mRNA levels by 46.1%. These findings highlight the critical role of OtTGR in parasite survival. Comparative with other trematodes, such as Schistosoma japonicum and Fasciola hepatica, suggests that OtTGR may serve as a promising target for vaccine or drug development. Although immune-protective studies were not feasible due to host incompatibility, the conserved role of TGR across trematodes underscores its potential for controlling orientobilharziasis. Future studies will explore the immunogenicity and protective efficacy of OtTGR to assess its candidacy as a therapeutic target.
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