N6-Methyladenosine Modification-Related Genes Express Differentially in Sterile Male Cattle-Yaks
文献类型： 外文期刊
作者： Liu, Yuxin 1 ; Chen, Lili 1 ; Jiang, Hui 5 ; Wang, Hongzhuang 5 ; Zhang, Yujiao 6 ; Yuan, Zhengrong 6 ; Ma, Yi 1 ;
作者机构： 1.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin 300381, Peoples R China
2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
3.Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin 300381, Peoples R China
4.Tianjin Engn Res Ctr Anim Hlth Farming, Tianjin 300381, Peoples R China
5.Xizang Acad Agr & Anim Husb Sci, Inst Anim Husb & Vet Med, Lhasa 850002, Peoples R China
6.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China
关键词： yak; cattle-yak; male sterility; m(6)A modification; m(6)A-related genes
期刊名称：LIFE-BASEL （ 影响因子：3.4； 五年影响因子：3.4 ）
ISSN：
年卷期： 2024 年 14 卷 9 期
页码：
收录情况： SCI
摘要： N-6-methyladenosine (m(6)A), an RNA post-transcriptional modification, plays a crucial role in spermatogenesis. Cattle-yaks are interspecific hybrid offsprings of yak and cattle, and male cattle-yaks are sterile. This study aims to investigate the role of m(6)A modification in male cattle-yak infertility. Herein, testicular tissues were analyzed via histological observations, immunohistochemical assays, reverse-transcription quantitative polymerase chain reaction, Western blotting, and immunofluorescence assays. The results revealed that male cattle-yaks presented smaller testes (5.933 +/- 0.4885 cm vs. 7.150 +/- 0.3937 cm), with only single cell layers in seminiferous tubules, and weakened signals of m(6)A regulators such as METTL14 (methyltransferase-like 14), ALKBH5 (alpha-ketoglutarate-dependent hydroxylase homolog 5), FTO (fat mass and obesity-associated protein), and YTHDF2 (YTH N-6-methyladenosine RNA binding protein F2), both at the RNA and protein levels, compared with those of yaks. Altogether, these findings suggest that m(6)A modification may play a crucial role in male cattle-yak sterility, providing a basis for future studies.
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