您好，欢迎访问西藏自治区农牧科学院 机构知识库！

CircRNA-5335 Regulates the Differentiation and Proliferation of Sheep Preadipocyte via the miR-125a-3p/STAT3 Pathway

文献类型： 外文期刊

作者： Guo, Wei 1 ; Ciwang, Renzeng 2 ; Wang, Lei 1 ; Zhang, Shuer 3 ; Liu, Nan 1 ; Zhao, Jinshan 1 ; Zhou, Lisheng 1 ; Li, Hegang 1 ; Gao, Xiaoxiao 1 ; He, Jianning 1 ;

作者机构： 1.Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Inst Anim Sci, Lhasa 850009, Peoples R China

3.Shandong Anim Husb Chief Stn, Jinan, Peoples R China

关键词： sheep; preadipocyte; STAT3; miR-125a-3p; circRNA-5335

期刊名称：VETERINARY SCIENCES （ 影响因子：2.4； ）

ISSN：

年卷期： 2024 年 11 卷 2 期

页码：

收录情况： SCI

摘要： Simple Summary Mutton is a popular source of high-quality protein and nutrients. It is considered a green food due to its low cholesterol levels and high nutritional value. Adequate intramuscular fat may increase marbling, decrease shearing forces, and improve meat taste and palatability. Intramuscular white fat cells are produced by the differentiation of preadipocytes. The content of intramuscular fat is directly related to meat quality. The influence of preadipocytes on intramuscular fat content is closely connected with the proliferation and differentiation of preadipocytes. Therefore, the study of precursor fat cells is becoming increasingly more detailed. Studying the expression of the circRNA-5335/miR-125a-3p/STAT3 network at the cellular and molecular levels is crucial to enhance mutton quality by improving fat formation.Abstract The content of intramuscular fat (IMF) from preadipocytes is proportional to meat quality in livestock. However, the roles of circRNAs in IMF deposition in sheep are not well known. In this study, we show that circRNA-5335/miR-125a-3p/STAT3 play a crucial adjective role in the proliferation and differentiation of sheep preadipocytes. In this study, we characterized the roles of differentially expressed circRNA-5335/miR-125a-3p/STAT3, which were screened from sheep of different months of age and based on sequencing data. Firstly, the expression profiles of circRNA-5335/miR-125a-3p/STAT3 were identified during the differentiation of preadipocytes in vitro by RT-qPCR and WB. Then, the targeting relationship of the circRNA-5335/miR-125a-3p/STAT3 was verified by dual-luciferase reporter assays. The results of RT-qPCR, CCK8, EdU and Oil Red O staining assay showed that miR-125a-3p suppressed the differentiation and raised the proliferation of preadipocytes by targeting STAT3. As a competing endogenous RNA, the downregulation of circRNA-5335 decreased the expression of STAT3 by increasing miR-125a-3p, which inhibited the differentiation of preadipocytes and promoted proliferation. Our present study demonstrates the functional significance of circRNA-5335/miR-125a-3p/STAT3 in the differentiation of sheep preadipocytes, and provides novel insights into exploring the mechanism of IMF.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：西藏自治区农牧科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台