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Optimization of Solid-State Fermentation Extraction of Inonotus hispidus Fruiting Body Melanin

文献类型： 外文期刊

作者： Zhang, Fengpei 1 ; Xue, Fanzheng 1 ; Xu, Hui 1 ; Yuan, Yuan 1 ; Wu, Xiaoping 1 ; Zhang, Junli 3 ; Fu, Junsheng 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China

2.Fujian Agr & Forestry Univ, Coll Life Sci, Mycol Res Ctr, Fuzhou 350002, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Lhasa 850000, Peoples R China

关键词： melanin; optimization; structure; Inonotus hispidus

期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）

ISSN：

年卷期： 2021 年 10 卷 12 期

页码：

收录情况： SCI

摘要： Melanin has good nutritional and medicinal value; however, its extraction rate is extremely low. This study explored the edible and medicinal fungus Inonotus hispidus fruiting body melanin (IHFM) extraction process and solid-state fermentation conditions. The results showed that the best way to extract IHFM is the compound enzymatic method, with complex enzyme 26.63 mg/g, liquid material ratio 5:1, enzymatic hydrolysis 80 min, pH 4.61, and enzymolysis temperature at 36.07 degrees C. The yield of IHFM was 23.73 +/- 0.57%, which was equivalent to 1.27 times before optimization. The best solid medium formula was normal pH, rice 20 g per cultivation bottle, maltose 22 g/L, beef extract 4.4 g/L, carbon-nitrogen ratio 5:1, and liquid-to-material ratio 1.1:1, where the IHFM yield was 31.80 +/- 1.34%, which was equivalent to 1.7 times that before optimization. In summary, solid-state fermentation and extraction optimization greatly improved the yield of melanin, provided a reference to produce melanin, and laid a foundation for the development and utilization of melanin.

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