您好,欢迎访问西藏自治区农牧科学院 机构知识库!

Identification, Nutrient Composition, and Evaluation of a Wild Pleurotus citrinopileatus Strain (X21156) from Tibet for Antioxidant and Cytotoxic Activities

文献类型: 外文期刊

作者: Xiao, Xiaoshan 1 ; Li, Yun 1 ; Li, Xiaomin 1 ; Hu, Xin 1 ; Zhang, Junli 2 ; Wu, Xiaoping 3 ; Fu, Junsheng 3 ;

作者机构: 1.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Lhasa 850000, Peoples R China

3.Fujian Agr & Forestry Univ, Coll Life Sci, Mycol Res Ctr, Fuzhou 350002, Peoples R China

关键词: Pleurotus citrinopileatus; fungi identification; biological properties; nutritional composition; antioxidant activities; cytotoxic effect

期刊名称:HORTICULTURAE ( 影响因子:3.1; 五年影响因子:3.4 )

ISSN:

年卷期: 2024 年 10 卷 4 期

页码:

收录情况: SCI

摘要: A fungal strain (X21156) collected in Tibet was used as the material, identified based on its morphological characteristics and internal transcribed spacer (ITS) sequence; its optimal culture conditions were analyzed by single-factor experiments; artificial domestication and cultivation were carried out; its nutrient composition was determined; and the bioactivities of its polysaccharides were detected using chemical antioxidant assays and MTT assays. The results showed that the strain was Pleurotus citrinopileatus Sing. Its optimal culture conditions were a pH of 7, a temperature of 25 degrees C, glucose (20 gL-1) as the carbon source, and yeast powder (20 gL-1) as the nitrogen source. The fresh weight of a single domesticated fruiting body was 41.16 g. The strain had high protein (28.5%), high fiber (34%), and low fat (1.4%) contents, with high proportions of fresh and sweet amino acids. Polysaccharides had good scavenging ability on ABTS+, DPPH, and OH free radicals (EC50 0.06 mg/mL, 1.21 mg/mL, and 3.62 mg/mL, respectively), and the cytotoxicity of polysaccharides to hepatocellular carcinoma cells (HepG2) (IC50: 1.69 mg/mL) was higher than that of triple-negative breast cancer cells (MDA-MB-468) (IC50: 1.76 mg/mL). In conclusion, the study provides a reference on the optimal culture conditions, domestication and cultivation, and dietary and medicinal values of wild P. citrinopileatus Sing.

  • 相关文献
作者其他论文 更多>>