Resistance to Powdery Mildew in Qingke Involves the Accumulation of Aromatic Phenolamides Through Jasmonate-Mediated Activation of Defense-Related Genes
文献类型： 外文期刊
作者： Xu, Congping 1 ; Zhan, Chuansong 3 ; Huang, Sishu 3 ; Xu, Qijun 1 ; Tang, Tang 5 ; Wang, Yulin 1 ; Luo, Jie 3 ; Zeng, Xingquan 1 ;
作者机构： 1.State Key Lab Hulless Barley & Yak Germplasm Resou, Lhasa, Peoples R China
2.Tibet Acad Agr & Anim Husb Sci Lhasa, Tibet, Peoples R China
3.Hainan Univ, Coll Trop Crops, Haikou, Peoples R China
4.Hainan Univ, Hainan Yazhou Bay Seed Lab, Sanya Nanfan Res Inst, Sanya, Peoples R China
5.Wuhan Metware Biotechnol Co Ltd, Wuhan, Peoples R China
6.Tibet Agr & Anim Husb Univ, Plant Sci Coll, Tibet, Peoples R China
关键词： powdery mildew (Blumeria graminis f; sp; hordei); metabolomics; transcriptome; proteome; jasmonic acid
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Powdery mildew (PM) leads to severe yield reduction in qingke (Hordeum vulgare L. var. nudum). Although studies have focused on identifying PM-related resistance genes, mechanistic insights into the metabolic regulation networks of resistance against PM have rarely been explored in qingke. Here, we integrated transcriptomic, proteomic and metabolomic data using PM-susceptible (G72) and PM-resistant (K69) accessions to systemically explore the mechanisms of PM resistance. The integrated results show that a rapidly transduction of jasmonic acid (JA) and (+)-7-iso-jasmonoyl-L-isoleucine (JA-Ile), and importantly, a inducing accumulation of aromatic PAs conferred qingke-specific resistance for PM stress. Functional analysis revealed that the four BAHD N-acyltransferase genes were responsible for the synthesis of aliphatic and aromatic PAs. The expression of the four genes are induced by methyl jasmonate (MeJA) and PM treatment. Co-expression network analysis shows that a histone lysine demethylase, JMJ705 gene, also induced by MeJA and PM treatment, had highly correlation with PAs biosynthesis. Chromatin immunoprecipitation (ChIP)-seq assays revealed that the level of trimethylated histone H3 lysine 27 (H3K27me3) of the four genes in MeJA and PM-treated plants was significantly reduced. Overall, our results suggest that a novel strategy for jasmonic acid signal-mediated demethylation controlling the accumulation of aromatic PAs to enhance plant immune resistance through removal of H3K27me3 and activating defense-related gene expression.
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