Expression of key genes involved in lipid deposition in intramuscular adipocytes of sheep under high glucose conditions
文献类型： 外文期刊
作者： Yan, Wei 1 ; Kan, Xiangdong 2 ; Wang, Yutao 3 ; Zhang, Yonghao 3 ;
作者机构： 1.Jiangsu Agrianim Husb Vocat Coll, Sch Anim Sci & Technol, Taizhou 225300, Peoples R China
2.Tibet Acad Agr & Anim Husb Sci, Inst Anim Husb & Vet Med, Lhasa, Peoples R China
3.Kashi Univ, Coll Life & Geog Sci, Kashi, Peoples R China
4.Key Lab Biol Resources & Ecol Pamirs Plateau Xinj, Kashi, Uygur Autonomou, Peoples R China
关键词： gene expression; intramuscular adipocytes; intramuscular fat; lipid metabolism
期刊名称：JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION （ 影响因子：2.718； 五年影响因子：2.747 ）
ISSN： 0931-2439
年卷期：
页码：
收录情况： SCI
摘要： The intramuscular fat (IMF) content in sheep is associated with IMF deposition, which is affected by intramuscular adipocyte hypertrophy. In this study, we established an in vitro high glucose model of intramuscular adipocytes of sheep to investigate the expression of cannabinoid receptor 1 (CB1) gene, fatty acid-binding protein 4 (FABP4) gene, lipid metabolism-associated genes (acetyl-CoA carboxylase [ACC], fatty acid synthase [FAS], and stearoyl-CoA desaturase 1 [SCD1]), and transcription factors (liver X receptor [LXR alpha]), sterol regulatory element-binding transcription factor 1 [SREBF-1], and carbohydrate-responsive element-binding protein [ChREBP]) as well as the changes in the lipid and triglyceride (TG) levels in intramuscular adipocytes. The results showed that the differentiated mature adipocytes had a spherical shape, and the number and volume of the lipid droplets gradually increased over time under high glucose conditions. The lipid and TG levels in intramuscular adipocytes of sheep continuously increased under high glucose conditions. Furthermore, CB1, FABP4, ACC, FAS, SCD1, LXR alpha, SREBF-1, and ChREBP were highly expressed under high glucose conditions, suggesting that the energetic nutrients also affect the expression of the CB1 gene, which works in coordination with lipid metabolism-associated genes and are beneficial for lipid deposition in the intramuscular adipocytes of sheep.
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