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Culture dependent and independent characterization of endophytic bacteria in the seeds of highland barley

文献类型： 外文期刊

作者： Chen, Yulan 1 ; Liang, Jinpeng 1 ; Zia, Alina 1 ; Gao, Xue 3 ; Wang, Yong 1 ; Zhang, Lingzi 1 ; Xiang, Quanju 1 ; Zhao, Ke 1 ; Yu, Xiumei 1 ; Chen, Qiang 1 ; Penttinen, Petri 1 ; Nyima, Tashi 3 ; Gu, Yunfu 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Resources, Dept Microbiol, Chengdu, Peoples R China

2.Liangshan Tobacco Corp Sichuan Prov, Xichang, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Inst Agr Resources & Environm Sci, Lhasa, Xizang, Peoples R China

关键词： Highland barley; endophytic bacteria; pure culture; 16S rRNA gene sequencing; plant growth promoting bacteria

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Endophytes in the seeds of plants have shown plant growth promoting (PGP) properties. Highland barley is an economically important crop and a major part of the local diet in the Tibetan Plateau, China, with potential health benefits. We applied culture-dependent and culture-independent methods to study endophytic bacteria in the seeds of eight Highland barley varieties. Based on the seed properties, the variety Ali was clearly separated from the other varieties except the variety CM. Most of the 86 isolates were assigned into genus Bacillus. Approximately half of the isolates showed PGP properties in vitro. Compared to the not-inoculated plants, inoculation with the isolate Bacillus tequilensis LZ-9 resulted in greater length and number of roots, and in bigger aboveground and root weights. Based on the 16S rRNA gene sequencing, the seed microbiome was majorly affiliated with the phylum Proteobacteria and the family Enterobacteriaceae. Overall, the bacterial community compositions in the different varieties were different from each other, yet the between variety differences in community composition seemed relatively small. The differences in community compositions were associated with differences in the total and reducing sugar contents and viscosity of the seeds, thus possibly connected to differences in the osmotic pressure tolerance of the endophytes. The results suggested that the seed endophytes are likely to promote the growth of Highland barley since germination.

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