西藏自治区农牧科学院机构知识库

Genetic diversity and classification of Tibetan yak populations based on the mtDNA COIII gene

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文献类型：外文期刊

作者： Song, Q. Q. ¹ ; Chai, Z. X. ¹ ; Xin, J. W. ³ ; Zhao, S. J. ¹ ; Ji, Q. M. ³ ; Zhang, C. F. ³ ; Ma, Z. J. ⁴ ; Zhong, J. C. ¹ ;

作者机构： 1.Southwest Univ Nationalities, Key Lab Anim Genet & Breeding, State Ethn Affairs Commiss, Chengdu, Peoples R China

2.Southwest Univ Nationalities, Minist Educ, Chengdu, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Inst Anim Sci & Vet, Lhasa, Peoples R China

4.Qinghai Acad Anim Sci & Vet Med, Inst Anim Sci, Xining, P

关键词： Mitochondrial DNA cytochrome c oxidase subunit 3;Genetic diversity;Phylogenetic relationship;Tibetan yak

期刊名称：GENETICS AND MOLECULAR RESEARCH （影响因子：0.764；五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 1 期

页码：

收录情况： SCI

摘要： To determine the level of genetic diversity and phylogenetic relationships among Tibetan yak populations, the mitochondrial DNA cytochrome c oxidase subunit 3 (COIII) genes of 378 yak individuals from 16 populations were analyzed in this study. The results showed that the length of cytochrome c oxidase subunit 3 gene sequences was 781 bp, with nucleotide frequencies of 29.2, 29.4, 26.1, and 15.2% for T, C, A, and G, respectively. A total of 26 haplotypes were identified, with 69 polymorphic sites, including 11 parsimony-informative sites and 58 single-nucleotide polymorphism sites. No deletions/insertions were found in sequence comparison, indicating that nucleotide mutation types were transitions and transversions. Haplotype and nucleotide diversities were 0.562 and 0.00138, respectively, indicating a high level of genetic diversity in Tibetan yak populations. Phylogenetic relationship analysis indicated that Tibetan yak populations are divided into 2 groups.

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