Immunogenicity and protective efficacy of a recombinant adenovirus containing the fusion protein of bovine parainfluenza virus type 3 genotype C in mice
文献类型： 外文期刊
作者： Zhang, Jiaqi 1 ; Zeng, Jiangyong 3 ; Yuan, Zhenjie 3 ; Huang, Xiangyue 4 ; Wu, Jinbo 4 ; Yu, Qisheng 1 ; Chen, Taoyun 1 ; Den, Gunan 1 ; Zhu, Chenxi 1 ; Zhang, Bin 1 ;
作者机构： 1.Southwest Minzu Univ, Coll Anim & Vet Sci, Chengdu 610041, Peoples R China
2.Minist Educ & Sichuan Prov Qinghai Tibetan Plateau, Key Lab, Chengdu 610041, Peoples R China
3.Tibet Acad Agr & Anim Husb Sci, Tibet Livestock Res Inst, Lhasa 850009, Peoples R China
4.Anim Husb Sci Inst ABa Autonomous Prefecture, Hongyuan, Peoples R China
5.Southwest Minzu Univ, Coll Anim & Vet Sci, Chengdu, Peoples R China
关键词： Bovine parainfluenza virus type 3 genotype C; Adenovirus vector vaccine; Fusion protein; Protection efficacy
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.8； 五年影响因子：4.0 ）
ISSN： 0882-4010
年卷期： 2023 年 185 卷
页码：
收录情况： SCI
摘要： Bovine parainfluenza virus type 3 (BPIV3) is a viral respiratory pathogen of cattle that causes substantial economic losses. A replicating-defective recombinant human adenovirus type 5 (HAd5), carrying a fusion protein of BPIV3 genotype C (HAd5-F), was constructed and evaluated for its immunogenicity and protective efficacy in mice. After intramuscular injection with the HAd5-F, the IgG titers against F proteins increased to 1:102,400, and virus-neutralizing titers increased to 1:256, significantly higher than those in the group injected with inactivated BPIV3C in mice (p<0.05). The splenic CD4(+)/CD8(+)T lymphocytes and IFN-gamma(+)/IL-4(+) cytokine percentages were more significant in the HAd5-F group than those in the control group. A BPIV3C challenge in a mouse model was used to assess protective efficacy of the HAd5-F. The viral loads in the lungs and tracheas of mice immunized with the HAd5-F were significantly lower than those in the control group (p<0.0001). There were no significant histopathological alterations in the lungs of mice vaccinated with the HAd5-F. These findings suggested that the HAd5-F elicited excellent immunity against BPIV3C infection.
- 相关文献
作者其他论文 更多>>
-
Study on molecular characteristics of Staphylococcus from yak milk-Xizang
作者：Meng, Fanxing;Zeng, Jiangyong;Ma, Hongcai;Wu, Qingxia
关键词：Yak milk;
Staphylococcus ; Antibiotic resistance; Resistance gene; Virulence gene; Molecular typing -
Prevalence and relative-risks of pasteurella in yaks of Xizang, China
作者：Ma, Hongcai;Wang, Dongjing;Yuan, Zhenjie;Shi, Bin;Zeng, Jiangyong;Meng, Fanxing;Zhao, Congming;Pan, Chang
关键词：ELISA;
Pasteurella ; Risk factor analysis; Seroepidemiology; Yak -
Characteristics and Differences of Gut Bacterial and Fungal Communities in Chickens with Different Breeds
作者：Yuan, Zhenjie;Wang, Yan;Wang, Yan;Almutairi, Mikhlid H.;Zakria, Hafiz Muhammad
关键词：Bacterial; Fungal; Gut Microbiota; Snowland white chickens; Tibetan chickens
-
Integrating network pharmacology, molecular docking, and molecular dynamics simulations to explore potential compounds and mechanisms of Coptis chinensis in treating streptococcal infections
作者：Qi, Wanxiang;Shi, Bin;Tang, Wenqiang;Zeng, Jiangyong;Zhuo, Ma;Ma, Hongcai
关键词：Streptococcus; Coptis chinensis; Network pharmacology; Molecular docking; Molecular dynamics
-
Appropriate Soil Fertilization or Drone-Based Foliar Zn Spraying Can Simultaneously Improve Yield and Micronutrient (Particularly for Zn) Nutritional Quality of Wheat Grains
作者：Gao, Xue;Zhao, Qiang;Zhu, Yinghua;Xia, Haiyong;Zhao, Qiang;Yuan, Nuo;Li, Xiaojing;Zhang, Bin;Kong, Lingan;Xia, Haiyong;Yuan, Nuo;Xia, Haiyong;Zhang, Bin;Kong, Lingan;Xia, Haiyong;Wang, Zhaohui;Xia, Haiyong;Wang, Zhaohui
关键词：zinc; micronutrient; macronutrient; agronomic biofortification; compound fertilizer; drone technology; foliar spraying
-
Changes in the Microbiome in Yak Mastitis: Insights Based on Full-Length 16S rRNA Sequencing
作者：Zhang, Lihong;Hu, Junjie;Ma, Hongcai;Tang, Wenqiang;Zeng, Jiangyong;Kulyar, Md. F.
关键词：mastitis; mammary gland; probiotics; 16S rRNA sequencing; yak
-
Gut microbiota in two chickens' breeds: Characteristics and dynamic changes
作者：Wang, Yan;Yuan, Zhenjie;Wang, Yan
关键词：Gut microbiota; Tibetan chickens; Diversity; Abundance
