文献类型： 外文期刊

作者： Xiao, Rui ¹ ; Zhang, Yongpeng ³ ; Zheng, Shuwen ¹ ; Shen, Yifan ¹ ; Yan, Yushan ¹ ; Li, Guoyong ¹ ; Li, Junyong ⁴ ; Yang, Zhongling ¹ ;

作者机构： 1.Henan Univ, Sch Life Sci, Int Joint Res Lab Global Change Ecol, Kaifeng, Henan, Peoples R China

2.Henan Univ, Xiaoqinling Ecol Restorat Field Observat & Res Stn, Kaifeng 475004, Peoples R China

3.Xizang Acad Agr, Anim Husb Sci XZAAAS, Agr Sci IAR, Nyingchi, Peoples R China

4.Henan Univ, Dabieshan Natl Observat & Res Field Stn Forest Eco, Kaifeng, Peoples R China

关键词： Alpine meadow; ammonia oxidizers; legacy effects; nitrification; nutrient addition; nutrient cessation; recovery

期刊名称：ECOSYSTEMS （ 影响因子：3.3； 五年影响因子：3.9 ）

ISSN： 1432-9840

年卷期： 2025 年 28 卷 5 期

页码：

收录情况： SCI

摘要： Nitrogen (N) input enhances N transformations, resulting in harmful environmental impacts by accelerating N loss. However, it remains unclear whether the effects of long-term N inputs on N transformations persist after N addition was ceased. We examined the changes in the communities and activities of ammonia oxidizers in an alpine meadow that underwent 15 years of nutrient addition followed by 7 years of nutrient cessation. Our results showed that plant N, soil total N, and available N returned to control levels seven years after nutrient addition stopped, whereas nitrification rates, mean N2O emissions, and ammonia-oxidizing bacteria (AOB) abundance remained elevated. Network complexity among AOB, ammonia-oxidizing archaea (AOA), and comammox Nitrospira was higher in nutrient cessation plots than in control plots. The elevated soil nitrification pattern under nutrient addition was primarily driven by high soil N availability from exogenous N inputs. In contrast, under nutrient cessation, enhanced microbial N cycling sustained the elevated soil nitrification pattern. Therefore, our findings suggest that direct measurements of soil and plant N may not sufficiently capture the recovery of N transformations, and that legacy effects of nutrient addition on N cycling microbes and their activities may persist for many years.