文献类型： 外文期刊

作者： Lu, Guilong ¹ ; Pan, Yong-Bao ³ ; Wang, Zhoutao ¹ ; Xu, Fu ¹ ; Cheng, Wei ¹ ; Huang, Xinge ¹ ; Ren, Hui ¹ ; Pang, Chao ¹ ; Que, Youxiong ¹ ; Xu, Liping ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Key Lab Sugarcane Biol & Genet Breeding, Minist Agr & Rural Affairs, Fuzhou, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Inst Vegetables, Lhasa, Peoples R China

3.ARS, Sugarcane Res Unit, USDA, Houma, LA USA

关键词： sugarcane; SNP chip; chlorophyll content; QTL mapping; candidate gene prediction

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Chlorophyll is the most important pigment for plant photosynthesis that plays an important role in crop growth and production. In this study, the chlorophyll content trait was explored to improve sugarcane yield. Two hundred and eighty-five F1 progenies from the cross YT93-159 x ROC22 with significantly different chlorophyll contents were included as test materials. The chlorophyll content of the C1 leaves during elongation phase was measured using a SPAD-502 meter through a three-crop cycle (plant cane, first ratoon, and second ratoon). Linkage analysis was conducted on a high-density genetic map constructed based on the sugarcane 100K SNP chip. In addition, Fv/Fm, plant height, stalk diameter, brix data were collected on plant cane during the elongation and maturation phases. The results showed that the C1 leaf SPAD values, which can be used as an important reference to evaluate the growth potential of sugarcane, were significantly and positively correlated with the Fv/Fm during elongation phase, as well as with plant height, stalk diameter, and brix during maturity phase (P < 0.01). The broad sense heritability (H2) of the chlorophyll content trait was 0.66 for plant cane crop, 0.67 for first ratoon crop, and 0.73 for second ratoon crop, respectively, indicating that this trait was mainly controlled by genetic factors. Thirty-one quantitative trait loci (QTL) were detected by QTL mapping. Among them, a major QTL, qCC-R1, could account for 12.95% of phenotypic variation explained (PVE), and the other 30 minor QTLs explained 2.37-7.99% PVE. Twenty candidate genes related to chlorophyll content were identified in the QTLs plus a 200-Kb extension region within either sides, of which four were homologous genes involved in the chlorophyll synthesis process and the remaining 16 played a certain role in chlorophyll catabolic pathway, chloroplast organization, or photosynthesis. These results provide a theoretical reference for analyzing the genetic mechanism of chlorophyll synthesis and subsequent improvement of photosynthetic characteristics in sugarcane.