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An improved high-quality genome assembly and annotation of Tibetan hulless barley

文献类型： 外文期刊

作者： Zeng, Xingquan 1 ; Xu, Tong 4 ; Ling, Zhihao 4 ; Wang, Yulin 1 ; Li, Xiangfeng 4 ; Xu, Shuqing 5 ; Xu, Qijun 1 ; Zha, San 1 ;

作者机构： 1.State Key Lab Hulless Barley & Yak Germplasm Res, Lhasa 850002, Tibet, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Res Inst Agr, Lhasa 850002, Tibet, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Lhasa 850002, Tibet, Peoples R China

4.Chengdu Life Baseline Technol Co LTD, Chengdu 610041, Peoples R China

5.Univ Munster, Inst Evolut & Biodivers, D-48149 Munster, Germany

期刊名称：SCIENTIFIC DATA （ 影响因子：6.444； 五年影响因子：9.051 ）

ISSN：

年卷期： 2020 年 7 卷 1 期

页码：

收录情况： SCI

摘要： Hulless barley (Hordeum vulgare L. var. nudum) is a barley variety that has loose husk cover of the caryopses. Because of the ease in processing and edibility, hulless barley has been locally cultivated and used as human food. For example, in Tibetan Plateau, hulless barley is the staple food for human and essential livestock feed. Although the draft genome of hulless barley has been sequenced, the assembly remains fragmented. Here, we reported an improved high-quality assembly and annotation of the Tibetan hulless barley genome using more than 67X PacBio long-reads. The N50 contig length of the new assembly is at least more than 19 times larger than other available barley assemblies. The new genome assembly also showed high gene completeness and high collinearity of genome synteny with the previously reported barley genome. The new genome assembly and annotation will not only remove major hurdles in genetic analysis and breeding of hulless barley, but will also serve as a key resource for studying barley genomics and genetics.

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