您好，欢迎访问西藏自治区农牧科学院 机构知识库！

Physiological interactions between the hypothalamic-pituitary-gonadal axis and spleen in rams actively immunized against GnRH

文献类型： 外文期刊

作者： Han, Xingfa 1 ; Ren, Xiaohua 1 ; Zeng, Yu 2 ; Zhou, Yuqin 1 ; Song, TianZeng 3 ; Cao, Xiaohan 1 ; Du, Xiaogang 1 ; Meng, 1 ;

作者机构： 1.Sichuan Agr Univ, Isotope Res Lab, Yaan 625014, Peoples R China

2.Sichuan Agr Univ, Coll Anim Sci, Chengdu Campus, Chengdu 611130, Sichuan, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Inst Anim Sci, Lhasa 850009, Peoples R China

4.Chinese Acad Agr Sci, Inst Anim Sci, Key Lab Farm Anim Genet Resources & Gennplasm Inn, Minist Agr, Beijing 100793, Peoples R China

关键词： Reproductive axis;Spleen;Immunocastration;GnRH;Immune cytokines;Ram

期刊名称：INTERNATIONAL IMMUNOPHARMACOLOGY （ 影响因子：4.932； 五年影响因子：4.624 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Hypothalamic-pituitary-gonadal (HPG) axis is strongly implicated in the regulation of immune system. The objective was to determine the effects of immunocastration on splenic reproduction- and immunity-related gene expressions, and serum cytokine profiles in rams. Forty rams were randomly allocated into three groups: control (n = 14); surgically castrated (n = 13); or immunized (n = 13) against 100 mu g D-Lys6-GnRH-tandem-dimer peptide conjugated to ovalbumin in Specol adjuvant at 6 months of age (with a booster 2 months later). Blood samples (for hormone and immune cytokine profiles) were collected at 1-month intervals until rams were slaughtered (10 months). Compared to intact controls, anti-GnRH immunization reduced (P < 0.05) serum concentrations of LH, FSH, and testosterone. Reduced testosterone abrogated its inhibitor feedback effect on the synthesis of GnRH in spleen, as evidenced by increased (P < 0.05) protein content and mRNA expressions of GnRH, and simultaneously decreased (P < 0.05) mRNA expressions of androgen receptor in spleen. In parallel with the increased GnRH production in spleen, the mRNA expressions of interleukin (IL)-2, IL-4, IL-6 and tumor necrosis factor alpha (TNF-alpha) as well as lymphocyte marker CD4, CD8 and CD19 molecules were increased (P < 0.05) in spleen. Consistently, serum concentrations of IL-2, IL-4, IL-6, TNF-alpha were increased (P < 0.05) in rams following immunization. Similarly, deprivation of testosterone by surgical castration also increased (P < 0.05) GnRH and thus immune cytokine expressions in spleen. Collectively, our data suggested that immunocastration increased GnRH production in spleen by abrogating the inhibitory feedback effects from testosterone, consequently improving the immune markers of spleen and serum immune cytokines in rams. (C) 2016 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Effect of immunization against GnRH on hypothalamic and testicular function in rams. Han, Xingfa,Gu, Longjun,Cao, Xiaohan,Du, Xiaogang,Zeng, Xianyin,Xia, Chenyang,Feng, Jing,Song, Tianzeng.

[2]GnRH主动免疫对雄性藏绵羊血液中甲状腺激素含量的影响. 宋天增,韩兴发,谭遥,刘亚成,曹晓涵,杜小刚,孟风艳,储明星,曾宪垠. 2016

[3]羊促性腺激素释放激素主动免疫去势的研究进展. 赵旺生,潘美兰,李海雁,陈晓英,曾宪垠,宋天增. 2023

[4]去势对雄性岗巴绵羊血液睾酮浓度和生产性能的影响. 宋天增,冯静,鲍宇红,元振杰,王燕,夏晨阳,马金英. 2015

[5]GnRH主动免疫对雄性藏绵羊血液中甲状腺激素含量的影响. 周玉芹,宋天增,韩兴发,谭遥,刘亚成,曹晓涵,杜小刚,孟风艳,储明星. 2017

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：西藏自治区农牧科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台