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Rare allele of HvLox-1 associated with lipoxygenase activity in barley (Hordeum vulgare L.)

文献类型： 外文期刊

作者： Guo, Ganggang 1 ; Dondup, Dawa 1 ; Yuan, Xingmiao 1 ; Gu, Fanghong 3 ; Wang, Deliang 3 ; Jia, Fengchao 4 ; Lin, Zhipin 1 ;

作者机构： 1.Chinese Acad Agr Sci, Key Lab Crop Germplasm Resources & Utilizat, Minist Agr,Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Lhasa 850032, Peoples R China

3.China Natl Res Inst Food & Fermentat Ind, Beijing 100027, Peoples R China

4.Beijing Yanjing Brewery Grp Co Ltd, Tech Res Ctr, Beijing 101300, Peoples R China

5.Beijing Yanjing Brew

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

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收录情况： SCI

摘要： Identification and allele-specific marker development of a functional SNP of HvLox - 1 which associated with barley lipoxygenase activity. Improving the stability of the flavor of beer is one of the main objectives in breeding barley for malting, and lipoxygenase-1 (LOX-1) is a key enzyme controlling this trait. In this study, a modified LOX activity assay was used for null LOX-1 mutant screening. Four barley landraces with no detected level of LOX-1 activity were screened from 1,083 barley germplasm accessions from China. The genomic sequence diversity of the HvLox-1 gene of the four null LOX-1 Chinese landraces was compared with that of a further 76 accessions. A total of 104 nucleotide polymorphisms were found, which contained 83 single-nucleotide polymorphisms (SNPs), 7 multiple-nucleotide polymorphisms, and 14 insertions and deletions. Most notably, we found a rare C/G mutation (SNP-61) in the second intron which led to null LOX-1 activity through an altered splicing acceptor site. In addition, an allele-specific polymerase chain reaction marker was developed for the genotyping of SNP-61, which could be used in breeding programs for barley to be used for malting. The objective was to improve beer quality

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