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Revealing the Fungi Microbiome Difference of Suffolk Cross with Tibetan Sheep on Plateau

文献类型: 外文期刊

作者: Ren, Yue 1 ; Zhaxi, Yangzhong 1 ; Liu, Mengjun 1 ; Idrees, Asif 3 ; Li, Kun 4 ;

作者机构: 1.Tibet Acad Agr & Anim Husb Sci, Inst Livestock Res, Lhasa 850000, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Anim Genet & Breeding Tibetan Plateau, Lanzhou, Peoples R China

3.KBCMA, Coll Vet & Anim Sci, Narowal, Pakistan

4.Nanjing Agr Univ, Inst Tradit Chinese Vet Med, Coll Vet Med, Nanjing 210095, Peoples R China

5.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China

关键词: Feeds; Fungi; Microbiome; Plateau sheep; Rumen

期刊名称:PAKISTAN VETERINARY JOURNAL ( 影响因子:2.3; 五年影响因子:1.5 )

ISSN: 0253-8318

年卷期: 2023 年 43 卷 4 期

页码:

收录情况: SCI

摘要: Sheep plays a vital role as ruminant animal, which contributes to human needs by supplying milk, mutton and cashmere. As lifestyle of people is improved, there is an increasing need for mutton and its products. Fungi are important component of the microbiota and may affect immune and inflammatory responses, digestion, and nutrients absorption. However, little information is available about the fungal microbiota in sheep on the plateau. Forty-five sheep were divided into five groups equally (n = 9) (AL, BL, CL, DL and EL), sheep in CL, DL and EL groups were given grain feeds twice daily. Sheep in AL were fed with alfalfa and oat grasses, and BL was offered with equal amount of alfalfa grass, oat grass and grain feeds. Samples of rumen fluid were collected from sheep for microbiome analysis after a four-month raising period. The microbiota analysis of collected rumen fluid samples revealed 2 333 664 filtered sequences and 3810 amplicon sequence variants (ASVs). Alpha diversity analysis showed obvious different chao1, Shannon_entropy and Simpson in sheep. A total of 7 phyla and 56 genera including potential beneficial fungi like Penicillium, Torula, Filobasidium, Tomentella and pathogenic Rhodotorula, etc. were significantly different in sheep. Our findings provide new insights of the diverse diets on the fungal microbiome of sheep and contribute for better feeding practices of sheep reared on the plateau.

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