文献类型： 外文期刊

作者： Gong, Jiahao ¹ ; Liu, Moxin ² ; Zuo, Runan ³ ; Song, Xinhao ⁴ ; Wang, Junqi ¹ ; Zuo, Qindan ¹ ; Jiang, Yan ⁵ ; Long, Yunfeng ⁵ ; Silang, Yuzhen ⁶ ; Luo, Zeng ⁶ ; Gao, Xiuge ¹ ; Guo, Dawei ¹ ;

作者机构： 1.Nanjing Agr Univ, Engn Ctr Innovat Vet Drugs, MOE Joint Int Res Lab Anim Hlth & Food Safety, Ctr Vet Drug Res & Evaluat,Coll Vet Med, 1 Weigang, Nanjing 210095, Peoples R China

2.Shanghai Customs Dist, 13 Zhongshan East Rd, Shanghai 200002, Peoples R China

3.Anhui Agr Univ, Coll Anim Sci & Technol, Anhui Prov Key Lab Vet Pathobiol & Dis Control, Anim Derived Food Safety Innovat Team, Hefei 230036, Peoples R China

4.China Pharmaceut Univ, Sch Tradit Chinese Pharm, Nanjing 211100, Peoples R China

5.Nanjing Customs Dist, Anim Plant & Food Inspect Ctr, 39 Chuangzhi Rd, Nanjing 210000, Peoples R China

6.Tibet Acad Agr & Anim Husb Sci, Inst Grassland Sci, 130 Jinzhu West Rd, Lhasa 850000, Peoples R China

关键词： Bacterial infection; Enrofloxacin; Nanostructured lipid carriers; Silver nanoparticles; Antibacterial; Synergistic therapy; Wound healing

期刊名称：INTERNATIONAL JOURNAL OF PHARMACEUTICS-X （ 影响因子：6.4； 五年影响因子：5.9 ）

ISSN：

年卷期： 2025 年 9 卷

页码：

收录情况： SCI

摘要： The colonization of bacterial pathogens is a major concern in wound infection and becoming a notable medical issue. Enrofloxacin (ENR) can be applied to treat skin infections, while poor water solubility and bioavailability limit its clinical application. Nanostructured lipid carriers (NLCs) enhance the solubility and bioavailability of drugs by encapsulating them, making them effective for the topical treatment of skin wound infections. Additionally, to enhance treatment efficacy and further improve wound healing, silver nanoparticles (AgNPs) were attached to the aforementioned matrix, which also improved its colloidal stability and reduced toxicity. Herein, a scalable poly (vinyl alcohol) modified NLCs-based antibacterial platform was fabricated by high-pressure homogenization method, to co-load ENR and AgNPs for treating the bacterial-infected wounds. The growth of common wound bacterial pathogens (Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa) was synergistically inhibited by released ENR and Ag+ from the poly (vinyl alcohol) modified enrofloxacin-silver composite nano-emulsion (ENR@PVA-NLCs/AgNPs). In the in vivo wound model, the Staphylococcus aureusinfected wound in rat almost completely disappeared after treatment with ENR@PVA-NLCs/AgNPs, and no suppuration symptom was observed. Importantly, this nanoplatform had negligible side effects in vivo. Taken together, the above results strongly demonstrate the promising potential of ENR@PVA-NLCs/AgNPs as a synergistic therapeutic agent for clinical wound infections.