您好，欢迎访问西藏自治区农牧科学院 机构知识库！

Identification of four heat shock protein genes and their expression in response to thermal stress in two strains of Neoseiulus barkeri (Acari: Phytoseiidae)

文献类型： 外文期刊

作者： Xu, Ya-Jing 1 ; Liu, Lei 1 ; Yang, Yang 1 ; Wang, Xian 1 ; Tian, Chuan-Bei 1 ; Li, Ya-Ying 1 ; Chen, Han-Qiu 2 ; Liu, Hua 1 ;

作者机构： 1.Southwest Univ, Coll Plant Protect, Key Lab Entomol & Pest Control Engn, Chongqing 400715, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Inst Vegetable, Lhasa 850032, Peoples R China

关键词： Predatory mite; heat shock protein; thermal stress; heat stress resistance; cold stress resistance

期刊名称：SYSTEMATIC AND APPLIED ACAROLOGY （ 影响因子：1.421； 五年影响因子：1.254 ）

ISSN： 1362-1971

年卷期： 2018 年 23 卷 4 期

页码：

收录情况： SCI

摘要： To explore the effects of heat shock proteins in the conventional strain (CS) and high temperature-adapted strain (HTAS) of Neoseiulus barkeri, we cloned and characterized the cDNA of the NbHsp40, NbHsp60, NbHsp70, and NbHsp90 genes and examined changes in expression levels with short-term exposure to heat or cold stress. All four of the genes showed highly conserved amino acid sequences and high homology to Galendromus occidentalis and N. cucumeris. Phylogenetic analysis of NbHsps indicated a high consistency with the known genes of model insects. These results demonstrate variations in the sensitivity of NbHsps to heat or cold stress; i.e., the expression of NbHsp60 did not change with the application of heat and cold stress, NbHsp90 and NbHsp40 were upregulated at high temperature compared to that at low temperature responses, and the expression of NbHsp70 increased with both heat and cold stress. Hsp expression also differed between the two strains: The application of a 40 degrees C heat shock resulted in a decrease in the expression of NbHsp40/70/90 in the CS within 1-2 h, but increased in the HTAS strain in 2 h. However, exposure to cold stress (0 degrees C and 5 degrees C) did not result in distinct changes in the expression of NbHsps in the CS, but a significant decrease in the HTAS. The expression of NbHsps further demonstrates that the HTAS has been acclimated to high temperatures. The results also determine that heat acclimation decreases cold tolerance in HTAS mites. This study reveals differences in the characteristics of heat and cold tolerance between the two strains of N. barkeri and provides useful information for future commercialization of N. barkeri for pest biological control, particularly the HTAS. Furthermore, it also describes high heat tolerance in HTAS at the molecular level.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：西藏自治区农牧科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台