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Genome-wide association analysis of fleece traits in Northwest Xizang white cashmere goat

文献类型： 外文期刊

作者： Lu, Xiaotian 1 ; Suo, Langda 1 ; Yan, Xiaochun 2 ; Li, Wenze 2 ; Su, Yixin 2 ; Zhou, Bohan 2 ; Liu, Can 2 ; Yang, Lepu 2 ; Wang, Jiayin 2 ; Ji, De 1 ; Cuomu, Renqing 1 ; Cuoji, Awang 1 ; Gui, Ba 1 ; Wang, Zhiying 2 ; Jiang, Wei 2 ; Wu, Yujiang 1 ; Su, Rui 2 ;

作者机构： 1.Xizang Acad Agr & Anim Husb Sci, Inst Anim Sci, Lhasa, Peoples R China

2.Inner Mongolia Agr Univ, Coll Anim Sci, Hohhot, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab Anim Genet & Breeding Tibetan Plateau, Lhasa, Peoples R China

4.Sino Arabian Joint Lab Sheep & Goat Germplasm Inn, Hohhot, Peoples R China

关键词： Northwest Xizang white cashmere goat; genome-wide association analysis; cashmere trait; GGP_Goat_70K SNP chip; linear mixed model; Sanger sequencing

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：2.9； 五年影响因子：3.3 ）

ISSN：

年卷期： 2024 年 11 卷

页码：

收录情况： SCI

摘要： Northwest Xizang White Cashmere Goat (NXWCG) is the first new breed of cashmere goat in the Xizang Autonomous Region. It has significant characteristics of extremely high fineness, gloss, and softness. Genome-wide association analysis is an effective biological method used to measure the consistency and correlation of genotype changes between two molecular markers in the genome. In addition, it can screen out the key genes affecting the complex traits of biological individuals. The aim of this study was to analyze the genetic mechanism of cashmere trait variation in NXWCG and to discover SNP locus and key genes closely related to traits such as superfine cashmere. Additionally, the key genes near the obtained significant SNPs were analyzed by gene function annotation and biological function mining. In this study, the phenotype data of the four traits (cashmere length, fiber length, cashmere diameter, and cashmere production) were collected. GGP_Goat_70K SNP chip was used for genotyping the ear tissue DNA of the experimental group. Subsequently, the association of phenotype data and genotype data was performed using Gemma-0.98.1 software. A linear mixed model was used for the association study. The results showed that four fleece traits were associated with 18 significant SNPs at the genome level and 232 SNPs at the chromosome level, through gene annotated from Capra hircus genome using assembly ARS1. A total of 107 candidate genes related to fleece traits were obtained. Combined with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis, we can find that CLNS1A, CCSER1, RPS6KC1, PRLR, KCNRG, KCNK9, and CLYBL can be used as important candidate genes for fleece traits of NXWCG. We used Sanger sequencing and suitability chi-square test to further verify the significant loci and candidate genes screened by GWAS, and the results show that the base mutations loci on the five candidate genes, CCSER1 (snp12579, 34,449,796, A -> G), RPS6KC1 (snp41503, 69,173,527, A -> G), KCNRG (snp41082, 67,134,820, G -> A), KCNK9 (14:78472665, 78,472,665, G -> A), and CLYBL (12: 9705753, 9,705,753, C -> T), significantly affect the fleece traits of NXWCG. The results provide a valuable basis for future research and contribute to a better understanding of the genetic structure variation of the goat.

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