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Enhanced biosorption of cadmium ions on immobilized surface-engineered yeast using cadmium-binding peptides

文献类型： 外文期刊

作者： Wang, Songting 1 ; Sun, Yongmei 1 ; Wang, Shihong 1 ; Fan, Chunkun 3 ; Wang, Daojie 1 ; Liu, Fei 1 ; Zhang, Haiyan 1 ;

作者机构： 1.Henan Univ, Sch Life Sci, Kaifeng, Peoples R China

2.Engn Res Ctr Appl Microbiol Henan Prov, Kaifeng, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Inst Agr Res, Tibet, Peoples R China

关键词： cadmium-binding peptide; phage peptide library; biosorption; surface-engineered yeast; immobilization

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）

ISSN：

年卷期： 2024 年 15 卷

页码：

收录情况： SCI

摘要： A new type of cadmium (Cd) ion cell surface adsorbent was developed by integrating bacteriophage display peptide library technology with cell surface display technology. Cd2+ chelating resin served as the target molecule in screening experiments, leading to the identification of four Cd2+ -binding peptides. These peptides were introduced into Saccharomyces cerevisiae via the pYD1 plasmid using lithium acetate heat shock transformation. Adsorption efficiency tests indicated that the engineered yeasts adsorbed more Cd2+ than the control strain EBY100 when exposed to the same amount of Cd2+. Among these peptides, sequence 3-containing strain was demonstrated to have the highest Cd2+ adsorption efficiency, being 35% higher than the control strain. Additionally, when this recombinant yeast strain was immobilized using sodium alginate, the adsorption efficiency was increased by 55.7% compared to the control strain.

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