文献类型： 外文期刊
作者： Qu, Wei 1 ; Xu, Yumin 1 ; Yang, Jing 1 ; Shi, Hanqing 1 ; Wang, Junli 2 ; Yu, Xinnai 1 ; Chen, Jiemin 1 ; Wang, Binyi 1 ; Zhuoga, Deqing 3 ; Luo, Mengcheng 1 ; Liu, Rong 1 ;
作者机构： 1.Wuhan Univ, TaiKang Med Sch, Sch Basic Med Sci, Hubei Prov Key Lab Dev Originated Dis, Wuhan 430071, Peoples R China
2.Youjiang Med Univ Nationalities, Affiliated Hosp, Reprod Med Ctr, Baise, Peoples R China
3.Tibet Acad Agr & Anim Husb Sci, Inst Livestock Res, Lhasa 850000, Peoples R China
关键词： berberine; spermatogenesis; gut microbiota; testosterone; ornithine metabolism
期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.4； 五年影响因子：3.5 ）
ISSN： 1672-9145
年卷期： 2025 年 57 卷 4 期
页码：
收录情况： SCI
摘要： Berberine (BBR) is used to treat diarrhea clinically. However, its reproductive toxicity is unclear. This study aims to investigate the impact of BBR on the male reproductive system. Intragastric BBR administration for 14 consecutive days results in a significant decrease in the serum testosterone concentration, epididymal sperm concentration, mating rate and fecundity of male mice. Testicular treatment with testosterone propionate (TP) partially reverses the damage caused by BBR to the male reproductive system. Mechanistically, the decrease in Muribaculaceae abundance in the gut microbiota of mice is the principal cause of the BBR-induced decrease in the sperm concentration. Both fecal microbiota transplantation (FMT) and polyethylene glycol (PEG) treatment demonstrate that Muribaculaceae is necessary for spermatogenesis. The intragastric administration of Muribaculaceae intestinale to BBR-treated mice restores the sperm concentration and testosterone levels. Metabolomic analysis reveals that BBR affects arginine and proline metabolism, of which ornithine level is downregulated. Combined analysis via 16S rRNA metagenomics sequencing and metabolomics shows that Muribaculaceae regulates ornithine level. The transcriptomic results of the testes indicate that the expressions of genes related to the low-density lipoprotein receptor (LDLR)-mediated testosterone synthesis pathway decrease after BBR administration. The transcriptional activity of the Ldlr gene in TM3 cells is increased with increased ornithine supplementation in the culture media, leading to increased testosterone synthesis. Overall, this study reveals an association between a BBR-induced decrease in Muribaculaceae abundance and defective spermatogenesis, providing a prospective therapeutic approach for addressing infertility-related decreases in serum testosterone triggered by changes in the gut microbiota composition.
- 相关文献
作者其他论文 更多>>
-
Advances in plant response to low-temperature stress
作者：Yu, Mingzhai;Wei, Xiaohong;Yu, Mingzhai;Luobu, Zhaxi;Zhuoga, Deqing;Tang, Yawei;Yu, Mingzhai;Luobu, Zhaxi;Zhuoga, Deqing;Tang, Yawei
关键词：Low-temperature stress; Physiological responses; Phytohormones; Transcriptional regulation;
CBFs -
Physiological and broadly targeted metabolomic analyses of barley (Hordeum vulgare L.) in response to low-temperature stress
作者：Yu, Mingzhai;Wei, Xiaohong;Yu, Mingzhai;Luobu, Zhaxi;Zhuoga, Deqing;Tang, Yawei;Yu, Mingzhai;Luobu, Zhaxi;Zhuoga, Deqing;Tang, Yawei;Wei, Xiaohong
关键词：Low-temperature; Barley; Physiology; Metabolome
-
Transcriptomic analysis reveals regulatory mechanisms of LncRNAs and mRNAs in hulless barley in response to cold stress
作者：Yu, Mingzhai;Zhuoga, Deqing;Zhuang, Dabin;Luobu, Zhaxi;Yu, Mingzhai;Zhuoga, Deqing;Zhuang, Dabin;Luobu, Zhaxi
关键词：Hulless barley; Cold stress; LncRNA; Transcriptome sequencing
-
Age-specific composition of milk microbiota in Tibetan sheep and goats
作者：Cao, Xi;Fang, Yumeng;Chen, Yulin;Zhang, Ke;Bandan, Pingcuo;Suo, Langda;Jiacuo, Gesang;Wu, Yujiang;Cuoji, Awang;Zhuoga, Deqing;Ji, De;Quzhen, Ciren
关键词：Milk microbiota; Ruminant; Colostrum; Milk composition; Lactobacillus
-
Silkworm pupa protein and its peptides: Preparation, biological activity, applications in foods, and advantages
作者：Wu, Xiaolin;Zhang, Yu;Wang, Chen;Chen, Xiaoyong;Suo, Huayi;Song, Jiajia;Yang, Jing;Mumby, William;Zhang, Yuhong
关键词：Silkworm pupae; Edible insects; Protein; Bioactive peptides; Biological activity
-
Comparative analysis of long noncoding RNA and mRNA expression provides insights into adaptation to hypoxia in Tibetan sheep
作者：Wang, Fan;Zeng, Qiaoying;Wang, Fan;Liu, Jianbin;Zhuoga, Deqing;Liu, Jianbin
关键词：
-
Different feeding strategies can affect growth performance and rumen functions in Gangba sheep as revealed by integrated transcriptome and microbiome analyses
作者：Jize, Zhang;Xiaoqing, Zhang;Na, Ta;Zhuoga, Deqing;Jiacuo, Gesang;Cuicheng, Luosang;Bandan, Pingcuo
关键词：feeding strategies; Gangba sheep; growth performance; transcriptome; microbiome
