Virus-induced silencing of genes encoding LEA protein in Tibetan hulless barley (Hordeum vulgare ssp vulgare) and their relationship to drought tolerance
文献类型： 外文期刊
作者： Liang, Junjun 1 ; Deng, Guangbing 1 ; Long, Hai 1 ; Pan, Zhifen 1 ; Wang, Chunping 1 ; Cai, Peng 3 ; Xu, Deling 1 ; Nima, Zha-Xi 5 ; Yu, Maoqun 1 ;
作者机构： 1.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Sichuan Agr Univ, Triticeae Res Inst, Yaan 625014, Peoples R China
4.China W Normal Univ, Coll Life Sci, Nanchong 637002, Peoples R China
5.Tibet Acad Agr & Anim Sci, Lhasa 850032, Peoples R China
6.Barley Improvement & Yak Breeding Key Lab Tibet A, Lhasa, Peoples R China
关键词： HVA1; Dhn6; Virus-induced gene silencing (VIGS); Barley stripe mosaic virus (BSMV); Water loss rate (WLR)
期刊名称：MOLECULAR BREEDING （ 影响因子：3.297； 五年影响因子：3.011 ）
ISSN： 1380-3743
年卷期： 2012 年 30 卷 1 期
页码：
收录情况： SCI
摘要： Expression of the late embryogenesis abundant (LEA) gene is usually associated with plant response to dehydration. In this study, a drought-tolerant genotype was screened from 48 accessions of Tibetan hulless barley (Hordeum vulgare ssp. vulgare). By using virus-induced gene silencing, the influence of two LEA genes (HVA1 and Dhn6) on drought tolerance of Tibetan hulless barley was investigated. Results of quantitative real-time PCR indicated that the relative expression levels of HVA1 and Dhn6 in silenced plants were significantly reduced compared with control plants. Both HVA1-silenced and Dhn6-silenced plants showed a consequently lower survival rate than control plants under drought stress. However, only HVA1-silenced plants exhibited a significantly higher water loss rate (WLR). These results suggested that HVA1 and Dhn6 might participate in adaptive responses to water deficit in different ways. Vegetative growth of HVA1-silenced plants was significantly retarded even under optimal growth conditions, and their biomass accumulation was also much lower than that of the controls. These results indicate that HVA1 might play a role in vegetative growth of Tibetan hulless barley.
- 相关文献
作者其他论文 更多>>
-
Divergent effects of β-glucan addition in baked vs steamed bread: Deciphering hydration of BG and its interaction with starch-protein networks
作者：Zhang, Zhihui;Zhou, Yonghong;Zhang, Zhihui;Pan, Zhifen;Li, Yutong;Zhao, Tianyi;Zhou, Qiang;Bian, Haoquan;Chen, Jiaxin;Wang, Tao;Li, Qiao;Li, Yutong;Zhao, Tianyi;Bian, Haoquan;Zhang, Yuhong;Zeng, Xingquan;Li, Zhongyi
关键词：Hull-less barley; beta-glucan; Water; Starch; Protein; Wheat
-
β-Glucan content increase in Waxy-mutated barley is closely associated with positive stress responses and is regulated by ASR1
作者：Li, Qiao;Pan, Zhifen;Zhang, Zhihui;Tang, Hongmei;Cai, Jingchi;Zhang, Zhihui;Cai, Jingchi;Zeng, Xingquan;Li, Zhongyi
关键词：beta-Glucan; Barley; Waxy; Stress; ASR1; CslF6
-
Extraction and characterization of waxy and normal barley beta-glucans and their effects on waxy and normal barley starch pasting and degradation properties and mash filtration rate
作者：Li, Qiao;Liu, Juan;Zhai, Huisheng;Zhang, Zhihui;Xie, Rong;Xiao, Futong;Pan, Zhifen;Zeng, Xingquan;Zhang, Yuhong;Li, Zhongyi
关键词：Normal hull-less barley; Waxy hull-less barley; beta-Glucan; Starch pasting; Starch digestion; Mash filtration
-
Effects of Two Starch Synthase Ila Isoforms on Grain Components and Other Grain Traits in Barley
作者：Pan, Zhifen;Deng, Xiaoqing;Li, Qiao;Xie, Rong;Zhai, Huisheng;Deng, Xiaoqing;Xie, Rong;Zeng, Xingquan;Luobu, Zhaxi;Tashi, Nyima;Zeng, Xingquan;Luobu, Zhaxi;Tashi, Nyima;Li, Zhongyi
关键词：barley; starch synthase Ha; mass spectrometry; Western blot; hardness
-
Identification of HvLRX, a new dehydration and light responsive gene in Tibetan hulless barley (Hordeum vulgare var. nudum)
作者：Liang, Junjun;Zhang, Haili;Yi, Ling;Long, Hai;Yu, Maoqun;Deng, Guangbing;Tang, Yawei
关键词：Tibetan hulless barley (Hordeum vulgare var; nudum); Light; Drought; BSMV; VIGS
-
Genetic and molecular characterization of determinant of six-rowed spike of barley carrying vrs1.a4
作者：Wang, Jin-Hui;Xu, Zhen-Mei;Qiu, Xue-Bing;Li, Li-Lan;Yu, Shui-Yang;Li, Tao;Tang, Yan-Yan;Pu, Xi;Zhang, Juan-Yu;Zhang, Hai-Li;Liang, Jun-Jun;Long, Hai;Deng, Guang-Bing;Wang, Jin-Hui;Li, Wei;Tang, Ya-Wei
关键词：
-
Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads
作者：Liu, Juan;Li, Qiao;Zhai, Huisheng;Pan, Zhifen;Liu, Juan;Zhang, Yuhong;Zeng, Xingquan;Tang, Yawei;Tashi, Nyima;Zeng, Xingquan;Tang, Yawei;Tashi, Nyima
关键词：bread; glycemic index; waxy hull-less barley; wholegrain; beta-glucan
