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CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective

文献类型： 外文期刊

作者： Song, Runjie 1 ; Zhai, Qing 2 ; Sun, Lu 1 ; Huang, Enxia 1 ; Zhang, Yu 1 ; Zhu, Yanli 1 ; Guo, Qingyun 3 ; Tian, Yanan 4 ; Z 1 ;

作者机构： 1.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Lhasa 850000, Tibet, Peoples R China

3.Qinghai Univ, Qinghai Acad Agr & Forestry Sci, Key Lab Agr Integrated Pest Management, Qinghai Prov State Key Lab Plateau Ecol & Agr, Xining 810016, Qinghai, Peoples R China

4.Texas A&M Univ, Coll Vet Med, Dept Vet Physiol & Pharmacol, College Stn, TX 77843 USA

关键词： Filamentous fungi; CRISPR; Cas9; Genome editing; Off-target

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2019 年 103 卷 17 期

页码：

收录情况： SCI

摘要： Filamentous fungi play an important role in human health and industrial/agricultural production. With the increasing number of full genomes available for fungal species, the study of filamentous fungi has brought about a wider range of genetic manipulation opportunities. However, the utilization of traditional methods to study fungi is time consuming and laborious. Recent rapid progress and wide application of a versatile genome editing technology, i.e., the CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-related nuclease 9) system, has revolutionized biological research and has many innovative applications in a wide range of fields showing great promise in research and application of filamentous fungi. In this review, we introduce the CRISPR/Cas9 genome editing technology focusing on its application in research of filamentous fungi and we discuss the general considerations of genome editing using CRISPR/Cas9 system illustrating vector construction, multiple editing strategies, technical consideration of different sizes of homology arms on genome editing efficiency, off-target effects, and different transformation methodologies. In addition, we discuss the challenges encountered using CRISPR/Cas9 technology and give the perspectives of future applications of CRISPR/Cas9 technology for basic research and practical application of filamentous fungi.

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