文献类型： 外文期刊

作者： Khan, Sawar ¹ ; Zhao, Xiaochao ¹ ; Hou, Yini ¹ ; Yuan, Chunxiu ¹ ; Li, Yumei ¹ ; Luo, Xiaoping ¹ ; Liu, Jianzhi ⁴ ; Feng, ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Key Lab Anim Parasitol, Minist Agr China, Shanghai 200241, Peoples R China

2.Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 250014, Peoples R China

3.Inner Mongolia Agr Univ, Coll Vet Med, Hohhot 010010, Inner Mongolia, Peoples R China

4.Tibet Acad Agr & Anim Husb Sci, Inst Anim Sci, Lhasa 850000, Tibet Autonomou, Peoples R China

关键词： 2b-RAD sequencing; genome-wide SNP analysis; Haemonchus contortus; selective sweeps; signature of selection

期刊名称：JOURNAL OF BIOSCIENCES （ 影响因子：1.826； 五年影响因子：2.107 ）

ISSN： 0250-5991

年卷期： 2019 年 44 卷 4 期

页码：

收录情况： SCI

摘要： The parasitic nematode Haemonchus contortus is one of the world's most important parasites of small ruminants that causes significant economic losses to the livestock sector. The population structure and selection in its various strains are poorly understood. No study so far compared its different populations using genome-wide data. Here, we focused on different geographic populations of H. contours from China (Tibet, TB; Hubei, HB; Inner Mongolia, IM; Sichuan, SC), UK and Australia (AS), using genome-wide population-genomic approaches, to explore genetic diversity, population structure and selection. We first performed next-generation high-throughput 2b RAD pool sequencing using Illumina technology, and identified single-nucleotide polymorphisms (SNPs) in all the strains. We identified 75,187 SNPs for TB, 82,271 for HB, 82,420 for IM, 79,803 for SC, 83,504 for AS and 78,747 for UK strain. The SNPs revealed low-nucleotide diversity (pi = 0.0092-0.0133) within each strain, and a significant differentiation level (average F-st = 0.34264) among them. Chinese populations TB and SC, along with the UK strain, were more divergent populations. Chinese populations IM and HB showed affinities to the Australian strain. We then analysed signature of selection and detected 44 (UK) and 03 (AS) private selective sweeps containing 49 and 05 genes, respectively. Finally, we performed the functional annotation of selective sweeps and proposed biological significance to signature of selection. Our data suggest that 2b-RAD pool sequencing can be used to assess the signature of selection in H. contortus.