文献类型： 外文期刊

作者： Dai, Xianglin ¹ ; Song, Dali ¹ ; Guo, Qiankun ¹ ; Zhou, Wei ¹ ; Liu, Guangrong ³ ; Ma, Ruiping ⁴ ; Liang, Guoqing ¹ ; He, ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Plant Nutr & Fertilizer, Beijing 100081, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Coastal Agr, Tangshan 063299, Peoples R China

3.Jiangxi Acad Agr Sci, Inst Soil Fertilizer & Resource Environm, Nanchang 330200, Jiangxi, Peoples R China

4.Tibet Acad Agr & Anim Husb Sci, Inst Resources & Environm, Lhasa 85000, Peoples R China

5.Int Plant Nutr Inst China Program, Beijing 100081, Peoples R China

关键词： Potential N fixation Rate; Diazotrophic co-occurrence; Keystone taxa patterns; Organic; Substitution fertilizer regime; Double rice cropping systems; Free-living nitrogen fixers

期刊名称：SOIL BIOLOGY & BIOCHEMISTRY （ 影响因子：5.795； 五年影响因子：6.767 ）

ISSN： 0038-0717

年卷期： 2021 年 156 卷

页码：

收录情况： SCI

摘要： Free-living nitrogen fixation (FLNF) by diazotrophs is a ubiquitous renewable resource and may constitute an attractive viable solution to the problem of environmental degradation caused by nitrogen over-fertilization. However, the biotic and abiotic control mechanisms of free-living nitrogen fixation are poorly understood. Moreover, there is rare information to link the composition and functions of diazotrophic communities; additionally, there are insufficient or inadequate predictors of the potential N fixation rate (PNFR) to help improve fertilization strategies. Here, through high-throughput sequencing, network analysis, and N-15(2) labelling methods, we investigated the long-term effects of partial substitution of mineral N by organic fertilizers on diazotroph abundance and composition in double rice cropping systems. The field trial was conducted for 34 years and included five treatments: control without fertilizer (CK); only mineral NPK fertilizer (NPK); 70% inorganic N + 30% organic N + PK (NPKM1); 50% inorganic N + 50% organic N + PK (NPKM2); 30% inorganic N + 70% organic N + PK (NPKM3). Our results revealed that at the same nutrient input level, increasing the percentage of organic N substitution reduced diazotrophic abundance. Compared with the CK and NPK treatments, the organic substitution regimes reduced the fluctuation range of the PNFR between the early and late rice growing seasons. NH4+-N was the primary factor that negatively correlated (p < 0.01) with diazotrophic abundance, while the potential N fixation rate was positively correlated (p < 0.01) with soil C/N. Among abiotic factors, the models incorporating soil C/N and Fe2+/Mo could accurately predict the potential N fixation rate. High-throughput sequencing and network analyses revealed that Bradyrhizobium and Geobacter were the dominant genera in diazotrophic co-occurrence and keystone taxa patterns under all fertilization regimes. Soil pH and NH4+-N were the most important factors in shaping the diazotrophic co-occurrence and keystone patterns in paddy soils, respectively. Diazotrophic keystone composition was more suitable for predicting and characterising the potential N fixation rate than the co-occurrence pattern of diazotrophs. Moreover, compared with the CK and NPK regimes, partial substitution of mineral N with organic N fertilizer improved the prediction accuracy of potential N fixation rate more effectively. Overall, we highlight the benefits of organic substitution fertilizer regime in stabilizing and improving the potential N fixation rate. Our findings provide relevant insights into the potential of key microbial taxa for predictive N fixation for optimising chemical N fertilizer application strategies in paddy soils.