文献类型： 外文期刊

作者： Jiang, Y. -L. ¹ ; Bao, W. -J. ¹ ; Liu, F. ¹ ; Wang, G. -S. ² ; Yurkov, A. M. ³ ; Ma, Q. ¹ ; Hu, Z. -D. ¹ ; Chen, X. -H. ¹ ; Zhao, W-N. ¹ ; Li, A. -H. ⁴ ; Wang, Q. -M. ¹ ;

作者机构： 1.Hebei Univ, Inst Life Sci & Green Dev, Sch Life Sci, Baoding 071002, Hebei, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Aquat Sci Inst, Lhasa 850000, Tibet, Peoples R China

3.Leibniz Inst DSMZ, German Collect Microorganisms & Cell Cultures, Braunschweig, Germany

4.Chinese Acad Sci, Inst Microbiol, China Gen Microbiol Culture Collect Ctr, Beijing 100101, Peoples R China

5.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China

6.Hebei Univ, Engn Res Ctr Microbial Breeding & Conservat Hebei, Baoding 071002, Hebei, Peoples R China

7.Hebei Univ, Hebei Basic Sci Ctr Biot Interact, Baoding 071002, Hebei, Peoples R China

关键词： Average nucleotide identity (ANI); basidiomycetous yeasts; genomic metrics; molecular phylogeny; seventy-eight new taxa.

期刊名称：STUDIES IN MYCOLOGY （ 影响因子：14.1； 五年影响因子：17.7 ）

ISSN： 0166-0616

年卷期： 2024 年 109 期

页码：

收录情况： SCI

摘要： More than 2 000 yeast strains isolated from 1 200 samples mostly collected from Tibet and Yunnan provinces in China were identified as 462 species according to the internal transcribed spacer including the 5.8S rDNA (ITS) and the D1/D2 domains of the large subunit rDNA (LSU) sequence analyses. Among them, 70 new basidiomycetous yeast species were proposed based on the multi-locus phylogenetic analyses including the D1/D2 domains, the ITS, the small subunit rDNA (SSU), the largest subunit of RNA polymerase II (RPB1), the second largest subunit of RNA polymerase II (RPB2) and translation elongation factor 1-alpha (TEF1), as well as the phenotypic comparisons. The average nucleotide identity (ANI) analysis with the genomic metric was also used in the evaluation of the species delimitation for the genera Holtermannia, Mrakia and Takashimella that contain closely related species with low sequence heterogeneity in both ITS and D1/D2 regions. Forty-six new species belonged to 16 genera in the Agaricomycotina, 13 new species occurred in 12 genera in the Pucciniomycotina, three new species were distributed in three genera in the Ustilaginomycotina, and eight new species were classified in seven newly established genera. One new family was also proposed based on one of these new genera. The analyses revealed several inaccurate species names attributed to genomes deposited in GenBank, indicating the necessity of a more rigorous quality checks of the genomes deposited in the public databases.