MicroRNA miR-212-5p Regulates the MEK/ERK Signaling Pathway by Targeting A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) to Regulate Cowshed PM2.5-Induced NR8383 Apoptosis
文献类型： 外文期刊
作者： Sun, Ke 1 ; Sun, Yize 1 ; Jia, Yunna 1 ; Duan, Xinran 1 ; Ma, Zhenhua 1 ; Zhang, Xiqing 1 ; Wang, Lixia 3 ; Zhu, Yanbin 2 ; Gao, Yunhang 1 ; Basang, Wangdui 2 ;
作者机构： 1.Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China
2.Tibet Acad Agr & Anim Husb Sci, Inst Anim Husb & Vet Med, Lhasa 850009, Peoples R China
3.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Changchun 130102, Peoples R China
关键词： cowshed PM2.5; miR-212-5p; mitochondrial damage; MEK/ERK signaling pathway; apoptosis
期刊名称：TOXICS （ 影响因子：4.6； 五年影响因子：4.8 ）
ISSN：
年卷期： 2023 年 11 卷 12 期
页码：
收录情况： SCI
摘要： Objective: To investigate the role of miR-212-5p-targeted ARAF during the apoptosis of rat alveolar macrophages induced by cowshed PM2.5. Methods: miRNA and related target genes and pathways were predicted using the KEGG, TargetScan, and other prediction websites. NR8383 macrophages were treated with cowshed PM2.5 to establish an in vitro lung injury model in rats; meanwhile, for the assessment of cell viability, apoptosis, intracellular calcium ions, and mitochondrial membrane potential in NR8383 cells, RT-qPCR was used to detect the expression of miR-212-5p and the target gene ARAF. Results: The bioinformatic analyses showed that miR-212-5p and ARAF were involved in PM2.5-associated cellular damage. Exposure to different concentrations (0 mu g/mL, 60 mu g/mL, 180 mu g/mL, 300 mu g/mL) with different durations (0 h, 12 h, 24 h, 48 h) of cowshed PM2.5 resulted in apoptosis, increased intracellular calcium ions, and decreased mitochondrial membrane potential. The miR-212-5p mimic group showed an up-regulation of Bax and cleaved Caspase 3 expression but decreased Bcl2 expression compared to the NC group, and overexpression of ARAF up-regulated the expression of p-MEK1/2 and p-ERK1/2 and simultaneously reversed the above phenomena. Conclusions: miR-212-5p targets ARAF to affect the cowshed PM2.5-induced apoptosis through the MEK/ERK signaling pathway, providing a potential target for relevant farming industry and pathology studies.
- 相关文献
作者其他论文 更多>>
-
Role of dietary energy and protein proportions on serum inflammatory factors, antioxidant capacity, and rumen microbiota in postpartum weaned female yak
作者：Zhu, Yanbin;Cidan, Yangji;Cisang, Zhuoma;Gusang, Deji;Danzeng, Quzha;Basang, Wangdui;Lu, Sijia;Ali, Munwar;Li, Kun;Saleem, Muhammad Usman
关键词：Postpartum weaned female yak; Rumen microbiota; 16S rRNA
-
Composition and diversity of rumen mycobiota in Jiani yaks (Bos grunniens jiani): insights into microbial ecology and functions
作者：Cidan, Yangji;Wang, Hongzhuang;Zhu, Yanbin;Basang, Wangdui;Cidan, Yangji;Wang, Hongzhuang;Zhu, Yanbin;Basang, Wangdui;Wang, Jia;Xu, Chang;Li, Kun;Khan, Muhammad Kasib
关键词：Yak; rib; gut; microbiota; mycobiota; ITS sequencing
-
Characterization of Enterococcus faecalis JM263 from the Yak Gut on the Qinghai-Tibet Plateau: A Probiotic Candidate with Strong Fiber-Degrading Capacity
作者：Cidan, Yangji;Cisang, Zhuoma;Liu, Guifang;Wu, Dan;Basang, Wangdui;Deji, Quzhen;Ciwang, Renzeng;Zhu, Yanbin;Kangji, Lamu;Zhou, Xinhong
关键词：
Enterococcus faecalis JM263; Yak; Cellulose degradation; In vitro rumen fermentation; Probiotic potential -
Joint exploration of network pharmacology and metabolomics on the effects of traditional Chinese medicine compounds in weaned yaks
作者：Lu, Sijia;Li, Kun;Zhu, Yanbin;Cidan, Yangji;Basang, Wangdui;Zhu, Yanbin;Zhang, Xiaojuan
关键词：traditional Chinese medicine compounds; weaned yaks; network pharmacology analysis; metabolomics; diarrhea
-
Traditional Chinese herbal formulas modulate inflammatory mediators, antioxidant enzyme levels, and ruminal microbiota composition in postpartum female Yaks
作者：Zhu, Yanbin;Cidan, Yangji;Wang, Hongzhuang;Basang, Wangdui;Wang, Jia;Ali, Munwar;Li, Kun;Zhang, Xiaojuan;Pubu, Panduo
关键词：Antioxidant; Rumen; 16S rRNA sequencing; Microbiome; Bos grunniens; Herbal
-
Evaluating the Effect of Dietary Protein-Energy Ratios on Yak Intestinal Microbiota Using High-Throughput 16S rRNA Gene Sequencing
作者：Zhu, Yanbin;Cidan, Yangji;Cisang, Zhuoma;Gusang, Deji;Danzeng, Quzha;Basang, Wangdui;Ali, Munwar;Lu, Sijia;Javed, Usama;Li, Kun
关键词：protein-energy ratio; 16S rRNA sequencing; microbial diversity; yak intestinal microbiota
-
Protective Effects of Traditional Chinese Herbal Medicine Formulas (TCHMFs) Via Influencing Anti-Oxidative Capacity, Inflammatory Mediators, and Gut Microbiota in Weaned Yaks
作者：Zhu, Yanbin;Cidan, Yangji;Basang, Wangdui;Zhu, Yanbin;Zhu, Yanbin;Lu, Sijia;Ali, Munwar;Li, Kun;Zhang, Xiaojuan;Pubu, Panduo;Kiani, Faisal Ayub;Saleem, Muhammad Usman
关键词：Bos grunniens; Intestinal microbiota; Serum parameters; Traditional Chinese Medicine; Weaning
